Original whole cell currents from hERG expressing HEK293 cells in the presence of a control internal solution and after the internal application of 1 mM TEA⁺. Solutions were exchanged 9 times (left).
Here, the hERG current amplitude (-40 mV) is plotted against the sweep count (time between sweeps: 15 s). The current level shows an overall rundown during the recording, but the cell is giga-sealed and stable throughout the complete experiment (right). The arrows indicate the traces plotted on the left figure.