The internal perfusion was used with Jurkat cells for internal application of Cs+-containing solution replacing the K+-containing control solution.
 
 
KV 1.3 currents recorded from Jurkat cells and block by Cs+. Top panel shows currents elicited by a voltage step from -80 to +40mV for 200ms, subsequent block of the current by internal perfusion of Cs+, and recovery after washout of Cs+. Internal solution replacement was repeated 19 times and the recording was stable for over 35 minutes.