The internal perfusion was used with Jurkat cells for internal application of Cs+-containing solution replacing the K+-containing control solution. |
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KV 1.3 currents recorded from Jurkat cells and block by Cs+. Top panel shows currents
elicited by a voltage step from -80 to +40mV for 200ms, subsequent block of the current
by internal perfusion of Cs+, and recovery after washout of Cs+. Internal solution
replacement was repeated 19 times and the recording was stable for over 35 minutes. |
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