Nanion Technologies - Port-a-Patch, Perfusion


	Nanion´s Perfusion System provides a fast and continuous perfusion. It allows accurate application of up to eight different compounds and solutions. The micro-controller based design makes it compatible with all commonly used patch clamp software solutions. Together with the dedicated perfusion chamber for the Port-a-Patch, it is a simple system for automated perfusion experiments. The system is fully software controlled, but can also be used in a manual mode. Furthermore, the system can be upgraded for temperature control.


Currents of RBL cells endogeneously expressing K⁺ permeable channels (holding potential -100 mV). The external K⁺ concentration was changed using the Perfusion System. Exchange times for all solution exchanges were approx. 150 ms. Low K⁺ (4.5 mM K⁺), high K⁺ (143 mM K⁺).
Product-Sheet (307kb)



Perfusion Chamber for the Port-a-Patch including a teflon manifold.		Control panel. The pump for aspirating the waste solution and the valves can be either controlled manually, by the electrophysiology software or by a standalone program.



The Perfusion System can be controlled by a variety of interfaces: Serial, analog or digital control connector. Direct connectors for the following interfaces are available: Digidata 1322, HEKA EPC-9/10, ITC 16-18.