A unique feature of the Patchliner is the internal solution exchange between two recordings. The figure shows two simultaneously recorded Jurkat cells in the presence of a control internal solution, after the exchange of the internal solution with a Cs⁺ containing solution and a subsequent washout step. The ability to change the internal solution multiple times, along with long lasting and stable whole cell recordings possible with the Patchliner opens up a broad new range of experiments.

Internal solution exchange. Since Kv1.3 is a K⁺ permeable channel the current can be decreased when the permeable K⁺ is replaced by the non-permeable cation Cs⁺.