• Vesicle Prep Pro

    First product on the market for automated preparation of solvent-free giant unilamellar vesicles (GUVs)
  • Vesicle Prep Pro

    GUVs are homogeneous in size - ideal for different applications

Vesicle Prep Pro - Liposomes made easy

The Vesicle Prep Pro is the first product on the market for automated preparation of solvent-free giant unilamellar vesicles (GUVs). The GUVs are formed by means of electro-swelling. The benefits the Vesicle Prep Pro offers include:

  • GUVs of homogeneous size (1 - 30 μm in diameter)
  • Temperature control
  • Adjustable parameters (temperature; current amplitude; frequency)
  • Variety of lipids can be used
  • Ideal for bilayer recordings and protein measurements
  • Stable and reproducible GUVs
  • Reusable ITO slides

The Vesicle Prep Pro has been designed to keep the preparation process simple. This ensures stable and reproducible results.

The chamber in which the GUVs are made is formed by ITO-coated glass cover slides so that vesicle formation can be monitored using a microscope.

Integrated features, including flexible protocol design and temperature control, allow generation of GUVs from a wide variety of lipids, e.g. lipids with high charge or high phase transition temperatures.

GUVs prepared with the Vesicle Prep Pro have proven ideal for investigations of biophysical properties of e.g. electrogenic transmembrane proteins, lipid raft formation, and protein-DNA interactions.

For detailed information:

VesicleControl: Software

VesicleControl: Software for the Vesicle Prep Pro device

VesicleControl big

Using VesicleControl, complex protocols for the Vesicle Prep Pro can be defined and visually inspected. Control of parameters such as voltage amplitude, frequency, temperature and time allow very flexible protocol design for the production of homogeneously-sized vesicles. In particular, due to the control over the temperature, it is possible to generate vesicles of homogeneous lipid distribution using lipids with different phase transition temperatures.

Buffers and Solutions

Buffers and Solutions for the Vesicle Prep Pro

The buffers and solutions for the Vesicle Prep Pro are produced by Nanion and an external partner, quality-assured in-house at Nanion headquarters and shipped from Munich to our international customers.


Available buffers and solutions
  • "Vesicle Prep Pro GUV Kit": 5 vials x 500 µL GUVs, 13x 100 mM HCl, 9x 1 mL Sorbitol, 1 mM EGTA, 2 mM HEPES (pH 7.0 KOH); 13x 200 mM KCl, 10 mM HEPES (pH 7.0 KOH); 14x 200 mM KCl, 10 mM Hepes (pH 4.0 KOH), 13x 200 mM NaCl, 10 mM Hepes (pH 7.0 NaOH); 14 x 200 mM NaCl, 10 mM Hepes (pH 4.0 NaOH) (Order # 051003)

Testimonials & Case Studies

Dr. Eric Stava - Statement about the Vesicle Prep Pro

icon vpp   “The Vesicle Prep Pro has been of great utility in our Bioelectronics research, allowing students and researchers with limited electrophysiology background to easily form giant unilamellar vesicles (GUVs).  The electronic control lets us alter the size and density of the GUVs, and the transparent chamber allows for excellent visualization of the electro-swelling process.”

Eric Stava, PhD, Research Assistant
University of Wisconsin-Madison, WI, US

Dr. Marisa Rangel - Statement about the Vesicle Prep Pro and Port-a-Patch

icon vpp   icon pap   “Our planar lipid bilayers are obtained from GUVs prepared with the Nanion Vesicle Prep Pro setup. Together with the Port-a-Patch, Nanion´s state-of-the-art automated patch clamp platform, the efficiency of characterization of pore-forming peptides (and other ionophores from natural sources) in artificial bilayers has greatly increased. Both systems are straightforward and easy to use, allowing high quality, low noise, single channel recordings.”

Marisa Rangel, PhD, Researcher 
University of Sao Paulo, Butantan Institute, Sao Paulo, Brazil, WI, US

Prof. Dr. Colin Taylor - Statement about the Port-a-Patch and Vesicle Prep Pro

icon pap   icon vpp   “We are using the Vesicle Prep Pro and Port-a-Patch in our laboratory to study recombinant channel proteins reconstituted in planar bilayers. The system is easy to use and it makes experiments very efficient. Besides the great equipment, Nanion, with their widespread expertise, is also providing excellent continuous support, helping with methodology optimization and problem solving.”

Colin Taylor, Professor of  Cellular Pharmacology, Department of Pharmacology
University of Cambridge, Cambridge, UK

Data and Applications

Alamethicin - Bilayer Recordings

application alameticin 2icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro data and applications:
Data were kindly provided by M. Sondermann/Prof. Behrends, Univ. Freiburg.

Alamethicin is a channel forming peptide and, when patch clamped, reveals multiple non-equidistant conductance levels due to formation of alamethicin oligomers in the bilayer. Alamethicin single channel conductances. Recordings from a GUV prepared bilayer in 85 mM KCl at -140 mV.

Alpha-Hemolysin - DNA Translocation

p45 4 AlphaHemolysinNeu icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro data and applications:

Data were kindly supplied by Prof. Fritz Simmel, Technical University of Munich, Munich, Germany.

Reconstituted Alpha hemolysin channels are constantly open at positive and negative membrane potentials. Gating is observed as a result of the passage of a single stranded DNA molecule through the pore. Recordings were performed on the Port-a-Patch.

Bilayer Recordings - Low Noise

application Low noise holdericon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro data and applications:

Typical recording of the Irms noise level of a bilayer (DPhPC) on the Low Capacitance Holder. Typical values are 220 fA

(10 kHz filter frequency, 50 kHz sampling rate. Cf. 2.3 pA RMS on the standard Port-a-Patch).

 

Cytolysin (Bacterial) - Bilayer Recordings

application bacterial 3icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro data and applications:
Recordings were kindly supplied by ITC & CNR-Istituto di Biofisica, Trento, Italy.

Traces were recorded in 100 mM KCl, 10 mM Hepes, 0.1 mM EDTA, pH 7 at -40 mV. Lipid: Diphtanoyl-PC.

 

Endoplasmatic Reticulum Membrane Protein Translocator (Sec61) - Single Channel recordings

Sec61 Rampicon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro data and applications:
Data were kindly provided by A. Cavalié, M. Jung, R. Zimmermann, Universität des Saarlandes, Homburg, Germany.

Microsomes were fused with GUVs within the VesiclePrepPro. The resulted Proteoliposomes formed a bilayer onto the NPC-1 Chip of the Port-a-Patch. Nice single channel openings could be recorded in symmetrical KCl solution applying a ramp from -110 mV to +100 mV for 400 ms.

Gramicidin - Single Channel Analysis

application gramicidin 1icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro data and applications:
Recordings were kindly supplied by Tohoku University, Tohoku, Japan.

Plotting the current amplitude vs. the voltage reveals conductances of 94.88 pS and 28.28 pS, which correspond to two different gramicidin derivates present in the bilayer. Traces were recorded in 100 mM HCl at the indicated potentials. Clearly two gramicidin derivates (94.88 pS and 28.28 pS) can be distinguished.

Gramicidin - Rapid Internal Solution Exchange

p24 4 InternExchicon pap    Port-a-Patch and   icon vpp   Vesicle Prep Pro data and applications:

Switching of internal solutions during gramicidin recordings from a lipid bilayer was obtained within seconds. A lipid bilayer was formed using giant unilamellar vesicles. Currents were recorded at a holding potential of +150 mV. The internal solution was switched from HCl to KCl, resulting in lower channel conductance.

GUVs - Average Diameter

p44 1 GUVicon vpp   Vesicle Prep Pro data and applications:
The Image was kindly provided of Dr. Sonderman/Prof. Behrends, University of Freiburg, Germany.

The GUVs produced with the Vesicle Prep Pro typically range from 1 – 30 μm in diameter, with an average diameter of
approximately 10 μm (60-70%).

 

Inositol-Triphosphate 3 Receptor (IP3) - Reconstitution

IP3 bea vereinfachticon vpp   Vesicle Prep Pro data and applications:
Data were kindly supplied by Prof. Colin Taylor, University of Cambridge, Cambridge, UK.

Representative traces of the IP3 (inositol-triphosphate) receptor were recorded at different voltages in the presence of IP3 (0.2 mM free Ca2+ and 1 mM Na2ATP, 140 mM KCl). Addition of 2 μM IP3 on the cytosolic side evoked openings of the IP3 receptor.

KcsA - Bilayer Recordings

KCSA Vesicle prep proicon vpp   Vesicle Prep Pro data and applicatons:

The image shows a representative current trace recorded from a planar lipid bilayer in which purified KcsA channels were reconstituted (100 mM KCl, asymmetric pH; pH 4/pH 7). A ramp from -100 mV to +100 mV was used to evoke the single channel events.

Download: Application Note

MscL - Pressure Activation

Vesicle prep pro MscLicon vpp   Vesicle Prep Pro data and applications:
Data were kindly supplied by Prof. Boris Martinac, University of QLD, Brisbane, Australia.

Pressure activation of MscL. The MscL channel is gated by pressure, where single channel activity was recorded at 30 mV at the indicated negative hydrostatic pressure. Nanion provides a dedicated suction control device for pressure clamp, found under the Port-a-Patch section.

MscL - Pressure Clamp Experiments

p16 2 MscLicon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro data and applications:

The Port-a-Patch and the Suction Control Pro was used to study the effect of pressure on functional MscL protein reconstituted in GUVs. Using symmetrical KCl solutions, MscL gating was observed at -30 mBar and -60 mBar (holding potential + 30 mV), shown as outward currents in the data traces.

OmpF - Bilayer Recordings

OMPFPolyamine beaicon vpp   Vesicle Prep Pro data and applications:
Data were kindly supplied by Prof. Mathias Winterhalter, Jacobs University, Bremen, Germany.

Channels were activated in the presence of spermine (0.5 mM). The downward spikes represent the closure events due to the effect of spermine. 

TRPM8 - Bilayer Recording

TrpM8 420x420icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro data and applications: 
Data kindly provided by Dr. Zakharian and Dr. Rohac From UMDNJ New Jersey Medical School, Newark, NJ 07103, USA

Shown are single channel events of TRPM8 reconstituted in a planar lipid bilayer. The recordings were made with the with the Port-a-Patch. The channel was activated by PIP2 and methanol. Currents were recorded at 100 mV.

Downloads:

Application Notes

KcsA - "Lipid bilayer recordings of KcsA channels reconstituted in proteoliposomes"

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro application note:  logo pdf   (0.2 MB)

MscL - "Lipid bilayer recordings of a mechanosensitive channel, MscL, using Nanion’s pressure clampchannel, MscL, using Nanion’s pressure clamp"

icon pap   Port-a-Patch and    icon vpp   Vesicle Prep Pro application note   logo pdf   (0.3 MB)

OmpF - "Lipid Bilayer recordings of OmpF reconstituted in Proteoliposomes "

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro application note:   logo pdf   (1.4 MB) 

Product Sheets

Vesicle Prep Pro - Product Sheet

icon vpp   Vesicle Prep Pro product sheet   logo pdf   (3.0 MB)

Publications

2017 - Polydim-I antimicrobial activity against MDR bacteria and its model membrane interaction

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in PLoS ONE (2017)

2017 - Organelle membrane derived patches: reshaping classical methods for new targets

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Nature Scientific Reports (2017)

2017 - Lipid phase separation in the presence of hydrocarbons in giant unilamellar vesicles

icon vpp   Vesicle Prep Pro publication in AIMS Biophysics (2017)

2017 - Ion- and water-binding sites inside an occluded hourglass pore of a trimeric intracellular cation (TRIC) channel

icon vpp   Vesicle Prep Pro publication in BioMed Central Biology (2017)

2017 - Evaluation of the antimicrobial activity of the mastoparan Polybia-MPII isolated from venom of the social wasp Pseudopolybia vespiceps testacea (Vespidae, Hymenoptera)

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in International Journal of Antimicrobial Agents (2017)

2017 - Engineering a pH responsive pore forming protein

Icon Orbit Mini  Orbit mini and   icon vpp   Vesicle Prep Pro publication in Scientific Reports (2017)

2017 - Bilayer-Mediated Structural Transitions Control Mechanosensitivity of the TREK-2 K2P Channel

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Structure (2017)

2017 - Asymmetric mechanosensitivity in a eukaryotic ion channel

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in PNAS (2017)

2016 - The N-terminal Ankyrin Repeat Domain Is Not Required for Electrophile and Heat Activation of the Purified Mosquito TRPA1 Receptor

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Nature (2016)

2016 - Structural and functional characterization of a calcium-activated cation channel from Tsukamurella paurometabola

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Nature Communications (2016)

2016 - Pore architecture of TRIC channels and insights into their gating mechanism

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Nature (2016)

2016 - Photosensitization in Porphyrias and Photodynamic Therapy Involves TRPA1 and TRPV1

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in The Journal of Neuroscience (2016)

2016 - Human TRPA1 is a heat sensor displaying intrinsic U-shaped thermosensitivity

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Scientific Reports (2016)

2016 - Functional characterization of p7 viroporin from hepatitis C virus produced in a cell-free expression system

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Protein Expression and Purification (2016)

2016 - Electrophysiological characterization of the archaeal transporter NCX_Mj using solid supported membrane technology

Icon N1  SURFE²R N1 and   icon vpp   Vesicle Prep Pro publication in Journal of General Physiology (2016)

2016 - Conductance and Capacity of Plain Lipid Membranes under Conditions of Variable Gravity

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Journal of Biomedical Science and Engineering (2016)

2015 - Quantification of Fluoroquinolone Uptake through the Outer Membrane Channel OmpF of Escherichia coli

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Journal of the American Chemical Society (2015)

2015 - High-Resolution Size-Discrimination of Single Nonionic Synthetic Polymers with a Highly Charged Biological Nanopore

Icon Orbit  Orbit 16 and   icon vpp   Vesicle Prep Pro publication in American Chemical Society Nano (2015)

2015 - Different Ligands of the TRPV3 Cation Channel Cause Distinct Conformational Changes As Revealed by Intrinsic Tryptophan Fluorescence Quenching

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Journal of Biological Chemistry (2015)

2015 - Cell-free expression of a functional pore-only sodium channel

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Protein Expression and Purification (2015)

2015 - Bilayer-Spanning DNA Nanopores with Voltage- Switching between Open and Closed State

Icon Orbit  Orbit 16 and   icon vpp   Vesicle Prep Pro publication in American Chemical Society Nano (2015)

2015 - Antibiotic translocation through porins studied in planar lipid bilayers using parallel platforms

Icon Orbit  Orbit 16,   icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Analyst (2015)

2014 - Temperature-sensitive gating of hCx26: high-resolution Raman spectroscopy sheds light on conformational changes

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Biomedical Optics Express (2014)

2014 - Studying mechanosensitive ion channels with an automated patch clamp

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in European Biophysics Journal (2014)

2014 - Structure of a Conserved Golgi Complex-targeting Signal in Coronavirus Envelope Proteins

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Journal of Biological Chemistry (2014)

2014 - Membrane assembly of the functional KcsA potassium channel in a vesicle-based eukaryotic cell-free translation system

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Biosensors and Bioelectronics (2014)

2014 - Human TRPA1 is intrinsically cold- and chemosensitive with and without its N-terminal ankyrin repeat domain

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Proceedings of the National Academy of Sciences of the United States of America (2014)

2014 - An outer membrane channel protein of Mycobacterium tuberculosis with exotoxin activity

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Proceedings of the National Academy of Sciences of the United States of America (2014)

2013 - Lipid-Bilayer-Spanning DNA Nanopores with a Bifunctional Porphyrin Anchor

icon vpp   Vesicle Prep Pro publication in Angewandte Chemie International Edition (2013)

2013 - Effect of flavonoids on the phase separation in giant unilamellar vesicles formed from binary lipid mixtures

icon vpp   Vesicle Prep Pro publication in Chemistry and Physics of Lipids (2013)

2012 - Natural and artificial ion channels for biosensing platforms

icon pap   Port-a-Patch,   icon pl   Patchliner,   icon sp96   SyncroPatch 96 ((a predecessor model of SyncroPatch 384PE) and   icon vpp   Vesicle Prep Pro publication in Analytical and Bioanalytical Chemistry (2012)

2012 - Hydrophobic gating of mechanosensitive channel of large conductance evidenced by single-subunit resolution

icon vpp   Vesicle Prep Pro publication in Proceedings of the National Academy of Sciences of the United States of America (2012)

2012 - Green Fluorescent Protein Changes the Conductance of Connexin 43 (Cx43) Hemichannels Reconstituted in Planar Lipid Bilayer

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in The Journal of Biological Chemistry (2012)

2012 - Design, synthesis and functional analysis of dansylated polytheonamide mimic: an artificial Peptide ion channel

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Journal of the American Chemical Society (2012)

2011 - Voltage-gated sodium channel (NaV) protein dissection creates a set of functional pore-only proteins

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Proceedings of the National Academy of Sciences of the United States (2011)

2010 - Studying mechanosensitive ion channels using liposomes

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Methods in Molecular Biology (2010)

2010 - Permeation of antibiotics through escherichia coli OmpF and OmpC porins: screening for influx on a single-molecule level

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Journal of Biomolecular Screening (2010)

2009 - The M34A mutant of Connexin26 reveals active conductance states in pore-suspending membranes

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Journal of Structural Biology (2009)

2009 - Amyloid-beta-Induced Ion Flux in Artificial Lipid Bilayers and Neuronal Cells: Resolving a Controversy

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Neurotoxicity Research (2009)

2008 - Rapid screening of membrane protein activity: electrophysiological analysis of OmpF reconstituted in proteoliposomes

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Lab-on-a-chip (2008)

2006 - High-resolution electrophysiology on a chip: Transient dynamics of alamethicin channel formation

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Biochimica et Biophysica Acta - Biomembranes (2006)

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Nanion Technologies GmbH

Ganghoferstr. 70A
D-80339 Munich - Germany
info@nanion.de