Nanion Technologies
23. - 27.09.2017 | SPS Annual Meeting

Venue: Maritim Hotel Berlin, Germany

Niels Fertig, Sonja Stölzle-Feix, Frank Henrichsen and Elena Dragicevic are looking forward to meeting you at our booth #206.

We will present five posters in collaboration with our partners Metrion Biosciences, University Medical Center Utrecht, Cellular Dynamics and  Clontech Takara Cellartis.

Posters:

"High Throughput Pharmacology of cardiac L-type Ca2+ Channels in overexpressing cell lines and iPSC-cardiomyocytes"
Download the abstract here.

"Cytotoxicity Monitoring for Safety Assessments: iPS Cardiomyocytes and Cancer Cells"
Download the abstract here.

"Differentiation and Validation of Human iPSC-Derived Atrial Cardiomyocytes"
Download the abstract here.

"Evaluation of Human iPS Cell-Derived Cardiomyocytes in High-Throughput Toxicity Screening Applications"
Download the abstract here.

"Integrating dynamic clamping with automated patch clamping devices: adding virtual IK1 channels to iPSC-CM"
Download the abstract here.


Do not miss our symposium in parallel to the SPS on Sunday 24th September, 4:00 PM - 6:30 PM  in the Maritim Hotel Salon 15 / Paris (1st floor).

Registration is free.

Title: HTS In Cardiac Safety

Eventbrite SPS Nanion Symposium Image

Find out more here.
_______ 

Go to the Conference website here.

24.09.2017 | HTS in Cardiac Safety Symposium

Venue: Maritim Hotel Berlin, Germany

Niels Fertig, Sonja Stölzle-Feix, Frank Henrichsen and Elena Dragicevic are looking forward to meeting you on Sunday 24th September, 4:00 PM - 6:30 PM in the Maritim Hotel Salon 15 / Paris (1st floor).

Title: HTS In Cardiac Safety

Eventbrite SPS Nanion Symposium Image

The Symposium is free to attend.

Register here.

_______

Confirmed speakers:

Said el Haou, Metrion Biosciences:
In depth profiling of human iPSC-CM – from electrophysiology to phenotypic assays"
Abstract:
tbd

Matt Burnham, AstraZeneca:
Impedance and field potential in 3D: cardiomyocyte co-culture spheroids and the CardioExcyte96 platform"
Abstract:
In vitro three-dimensional and co-culture cell models can provide more physiologically relevant data than conventional 2D approaches. We explored novel label-free methods to record functional data from actively beating, 3D spheroid co-cultures of iPSC cardiomyocytes. Using the Nanion CardioExcyte® 96 platform, designed for recordings from 2D iPSC cardiomyocytes monolayers, the electrical activity (extracellular field potential) and beating pattern (impedance) of cardiomyocyte spheroids could be detected. To facilitate the essential spheroid-to-electrode positioning, magnetic nano-particle based approaches were explored. Treatment with compounds known to affect beat rate and field potential duration generated appropriate results in the 3D system. A cumulative-dosing approach was developed to demonstrate a drug discovery work flow suitable for profiling studies. In comparison to a conventional contractility assay approach (2D FLIPR), significant advantages in reduced iPSC usage and cost (approximately eight-fold) and increased through-put were demonstrated. The high through-put of the system was leveraged to profile the functional phenotype of various iPSC and fibroblast co-culture types under different experimental conditions. Phenotype profiling revealed substantial differences amongst co-culture types, such as in beat amplitude and responses to E-4031. Electrical pacing revealed true inotropic responses (increased beat amplitude at constant beat rate) in some conditions. In summary, recording functional data from the 3D phenotype of spheroid co-cultures of electrically-active and beating cardiomyocytes in a screening platform has been achieved which may improve the predictivity of Safety assays and facilitate a better understanding of 3D co-culture phenotypes.

 Takasuna Kiyoshi, Daiichi Sankyo/ Novare:
Comprehensive in vitro cardiac safety assessment using human stem cell technology (CSAHi: HEART initiative) -The second stage"
1) Discovery Pharmacokinetics Research Group, Pharmaceutical and Biomedical Analysis Department, Daiichi Sankyo Rd Novare Co., Ltd.
2) Consortium for Safety Assessment using Human iPS Cells (CSAHi): HEART Team
Abstract:
Since 2013, we have organized the Consortium for Safety Assessment using Human iPS Cells (CSAHi; http://csahi.org/en/) in Japan to verify the application of human iPS/ES cell-derived cardiomyocytes in drug safety evaluation. The definitive goal of the CSAHi HEART Team has been to propose comprehensive screening strategies to predict a diverse range of cardiotoxicities (arrhythmia, contractile dysfunction, and cardiomyocyte toxicity) by using recently introduced platforms (multi-electrode array [MEA], patch clamp, cellular impedance, motion field imaging [MFI], and Ca transient systems) while identifying the strengths and weaknesses of each.
Our study (the first stage) showed that hiPS-CMs used in these platforms would offer paradigm changes of platforms for predicting drug-induced QT risk and/or arrhythmia or contractile dysfunction with a greater prognostic value beyond existing indirect surrogates proposed in animal models. I will introduce the latest progress and roadmap of activities of the CSAHi HEART Team (the second stage).

Teun de Boer, University of Utrecht:
Dynamic clamping on a Patchliner: adding virtual IK1 channels to cardiomyocytes"
Department of Medical Physiology, Division Heart & Lungs, University Medical Center Utrecht. Yalelaan 50, 3584 CM Utrecht, The Netherlands
This email address is being protected from spambots. You need JavaScript enabled to view it. Abstract:
Human pluripotent stem cell-derived cardiomyocytes (hPSC-CM) are an interesting source of cells for safety pharmacology, but there are caveats that have to be taken into account. A typical property of hPSC-CM is automaticity, the cardiomyocytes beat spontaneously, resembling cells in the sinoatrial node of the heart. The spontaneous beating is due to lack of IK1 ion channels, which normally assure a stable resting membrane potential in cardiomyocytes. Work by our group has demonstrated that the depolarized state of hPSC-CM limits their usefulness in assays aimed at detecting proarrhythmic properties of drugs (Jonsson et al., 2012, PMID: 22353256).
Dynamic clamping can provide virtual, simulated IK1 channels to a real biological hPSC-CM in a patch clamping experiment. Key benefits of this approach include full control of the added IK1 conductance to each cardiomyocyte, it can be applied to any hPSC-CM source and it can be coupled to automated patch clamping machines. In this webinar I will discuss our work on using the dynamic clamping technique with a Patchliner.

28.09.2017 | iForum User Meeting

Venue: Imperial College London, UK

Elena Dragicevic is looking forward to meeting you.

Elena will give an oral presentation:

"Holistic view on state-of-the-art cardiac safety screening"
Download the abstract here.

Go to the iForum website here.

03. - 04.10.2017 | ELRIG Drug Discovery

Venue: ACC Liverpool, UK

Alison Obergrussberger is looking forward to meeting you.

Ali will present three posters:

"Newest Advances in Automated Patch Clamp: Combining flexibility with high throughput"
Download the abstract here.

"Investigations into Idiosyncratic Drug-Induced Hepatotoxicity and Chronic Proliferation of Cancer Cells using a Label-free Method"
Download the abstract here.

"Targeting transporters with HTS electrophysiology on the SURFE²R 96SE"
Download the abstract here.

Go to the Conference website here.

10. - 13.10.2017 | Biophysics Mexico

Emerging Concepts in Ion Channel Biophysics

Venue:  Old Palace of Medicine, National Autonomous University of Mexico, Mexico City, Mexico


The team of Nanion would like to state the following concerning the latest news which have come to our attention:
"Our hearts go out to those affected by the recent earthquake in Mexico. Tireless search and rescue efforts are still ongoing, with many people still trapped under rubble in and around Mexico City. It is unclear at this stage whether this conference will take place, and if Nanion will attend due to these tragic circumstances. We wish the search and rescue teams all the best and hope for news of more survivors."


In case Nanion will attend, Andrea Brüggemann will give an oral presentation:

Tuesday, October 10, 2017, Session II: Ion Channels in Physiology, 1:55 PM - 2:15 PM.

Title: "News and Views on Cardiac Safety"


View the Conference program here.

Go to the Conference website here.

25. - 26.10.2017 | 8th Annual Nanion User Meeting Munich

Venue: Ganghoferstr. 70a, Munich, Germany

_______

Confirmed speaker:

 

Thomas Licher, Sanofi Frankfurt, Germany

Lars Kästner, Saarland University, Germany

Arne Hansen, UKT Hamburg, Germany

Oliver Reinhardt, MPI for Experimental Medicine, Göttingen, Germany

Tobias Brügmann, University of Bonn, Germany

Herbert Himmel, Bayer, Wuppertal, Germany

Dan Klærke, University of Copenhagen

_______ 

More information and the agenda will follow soon.

Click here to read the booklet of the 7th User Meeting in 2016.

_______

Registration:

The event is free. If you are interested to attend register here.

Download here the flyer for information on recommended hotels and public transportation to the headquarters.

(Please note spaces are limited and Nanion reserves the right to give preference to existing clients.)

_______

Posters and oral presentations:

As last year, we are organising a symposium and a poster session. If you are interested to present your data, please send an e-mail with your title and abstract to: This email address is being protected from spambots. You need JavaScript enabled to view it.