Shown is a raw current response of a HEK293 cell expressing AChR (α₃β₄) to exposure to 300 μM nicotine. Solutions were  stacked (layered) in the pipette to achieve brief exposure times.

Increasing acetylcholine concentrations (30 µM, 100 µM and 300 µM) were added to the same cell using a stacked applications protocol.
Cells were kindly provided by Galantos Pharma GmbH.

22 selected compounds were tested in a blind study on the SyncroPatch 96 using HEK 293 cells expressing hNav1.5 ion channels. The IC50-values were compared to manual patch clamp measurements, performed at the customer site.

Online analysis results of the dose response curves were obtained with a few mouse clicks. The color code of the online analysis helps to visualize different concentrations applied.
The corresponding data traces is shown in the data set "GABA Dose Response Curves".

Increasing concentrations of GABA were applied to each cell. Cells were from frozen stocks and were prepared for a FliPR experiment running alongside.
The corresponding online analysis of this experiment is shown in the data set "GABA CRC Analysis".

Shown is a raw current response of a HEK293 cell expressing GABA_A receptors to initial exposure to GABA, followed by joint exposure to GABA and diazepam (as indicated). Solutions were  stacked (layered) in the pipette to achieve brief exposure times.

With the SyncroPatch^® 96 currents can be recorded continuously during compound application. This, in conjunction with a solution exchange time of about 100 ms, makes the SyncroPatch^®96 an excellent tool for investigations of ligand gated ion channels. In the data example, 10 μM GABA was added to a HEK293 cell expressing GABA_A (α1β2γ2) receptors. The boxed trace shows a close up of the GABA-response.

Shown are raw current responses of HEK293 cells expressing hNa_V1.5 to a double (inactivation) pulse protocol.

Cells were kindly provided by Cytomyx Millipore, UK.

Timecourse of the Na_V1.5 peak currents in response to exposure to different lidocaine concentrations (0 μM, 1 μM, 10 μM, 100 μM, 0 μM). Time points at which the external solution was exchanged is marked by the red lines.

Cells were kindly provided by Cytomyx Millipore, UK.

The SyncroPatch^® 96 supports internal perfusion allowing internal administration of compounds, second messengers and metabolites. Here, K_V1.3 currents, endogenously expressed in Jurkat cells, were blocked by the internal administration of Cs⁺ followed by washout with Cs⁺-free internal solution.

Shown is a screenshot made during a recording of K_V1.3 currents stably expressed in CHO cells. On the top left the raw currents from each individual cell are displayed. The background color coding marks the cells with seals above 500 MΩ in blue and above 1 GΩ in green.

Cells were kindly provided by Evotec AG, Hamburg, Germany.

Inhibition of Nav1.5 currents in stem cell-derived cardiomyocytes (iCells) by lidocaine.  Lidocaine concentrations: 6 µM, 62 µM and 620 µM. The obatined  IC₅₀ -value was 14 µM.

Cells were kindly supplied by CDI.

Human nicotinic ACh receptors were activated by 100 µM ACh. Solution switch time was fast enough to see nAChR a7 responses.

Shown are three consecutive current responses of 1321 N1 cells expressing P2X2/3 receptors to 10 µM ATP. Cells were washed twice between compound applications (holding −80 mV). The data demonstrate the reproducability of the whole cell current responses.

Cells were kindly supplied by Evotec AG, Hamburg, Germany

After the desensitization of the nAChR a7 receptor by 100 µM acetylcholine, 10 µM PNU-120596 was applied together with 100 µM of Ach, reactivating the ion channels.

Cells were kindly provided by Galantos Pharma GmbH.

These statistics summarize experiments conducted with HEK293, CHO, Ltk, RBL, and Jurkat cells. More than 50 % of the cells have a seal resistance above 1 GΩ. In total more than 80 % of the cells had a whole cell resistance above 0.5 GΩ.

The traces show repeated application of 100 µM GABA to GABA_A receptors (α1 β2 γ2) expressed in HEK 293 cells. Current amplitudes were stable over time, illustrating the reliable and fast solution excahnge supported by the SyncroPatch 96.

Using the SyncroPatch^® 96 CHO cells expressing TRPV1 were subjected to a voltage ramp (-100 mV to +100 mV) in the presence and absence (control) of 2 μM capsaicin. As shown in the exemplar traces, inward and outward currents increase upon capsaicin addition.