Data and Applications
Bilayer Recordings on the Patchliner
With suction the GUVs are attracted to the aperture. As soon as one GUV hits the glass substrate, it bursts and forms a bilayer across the aperture. Shown are single channel recordings from gramicidin which was incorprated into the bilayer after its formation. Traces were recorded in 100 mM HCl at −100 mV.
Bilayer Recordings of KcsA
The image shows a representative current trace recorded from a planar lipid bilayer in which purified KcsA channels were reconstituted (100 mM KCl, asymmetric pH; pH 4/pH 7). A ramp from -100 mV to +100 mV was used to evoke the single channel events.
Download: Application Note
Bilayer Recordings of OmpF
Channels were activated in the presence of spermine (0.5 mM). The downward spikes represent the closure events due to the effect of spermine.
Data were kindly supplied by Prof. Mathias Winterhalter, Jacobs University, Bremen, Germany.
DNA Translocation in Hemolysin
Reconstituted Alpha hemolysin channels are constantly open at positive and negative membrane potentials. Gating is observed as a result of the passage of a single stranded DNA molecule through the pore. Recordings were performed on the Port-a-Patch®.
Data were kindly supplied by Prof. Fritz Simmel, Technical University of Munich, Munich, Germany.
GUVs
The GUVs produced with the Vesicle Prep Pro® typically range from 1 – 30 μm in diameter, with an average diameter of
approximately 10 μm (60-70%).
The Image was kindly provided of Dr. Sonderman/Prof. Behrends, University of Freiburg, Germany.
Pressure Activation of MscL
Pressure activation of MscL. The MscL channel is gated by pressure, where single channel activity was recorded at 30 mV at the indicated negative hydrostatic pressure. Nanion provides a dedicated suction control device for pressure clamp, found under the Port-a-Patch® section.
Data were kindly supplied by Prof. Boris Martinac, University of QLD, Brisbane, Australia.
Reconstitution of IP3 Receptors
Representative traces of the IP3 (inositol-triphosphate) receptor were recorded at different voltages in the presence of IP3 (0.2 mM free Ca2+ and 1 mM Na2ATP, 140 mM KCl). Addition of 2 μM IP3 on the cytosolic side evoked openings of the IP3 receptor.
Data were kindly supplied by Prof. Colin Taylor, University of Cambridge, Cambridge, UK.
TRPM8
Shown are single channel events of TRPM8 reconstituted in a planar lipid bilayer. The recordings were made with the with the Port-a-Patch®. The channel was activated by PIP2 and methanol. Currents were recorded at 100 mV.
Data kindly provided by Dr. Zakharian and Dr. Rohac From UMDNJ New Jersey Medical School, Newark, NJ 07103, USA