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2019 - SURFE²R as a service - closing the gap between technology and application

Icon 96SE   SURFE²R 96SE Oral Presentation

Presenter: 
Dr. Ralf Schwandner, CEO of Assay.Works GmbH, Germany
Assay.Works offers assay and screening services using the SURFE²R 96SE. Find out more here.

Source:
10th Nanion User Meeting, Munich, Germany

October 17, 2019


Abstract:

Solid-supported membrane (SSM)-based electrophysiology allows functional characterization of transporters which are otherwise difficult to investigate due to their slow transport conductivity or intracellular localization. The "SURFE²R 96SE" platform makes this technology suitable for compound characterization at higher throughputs.
For this case study, we prepared membrane vesicles from a PepT1-expressing cell line and screened an ion-channel/transporter-focused library of 237 bioactive compounds for substrate and inhibitory activity. With the SURFE²R 96SE, the screen was completed in less than 8 hours with a success rate of 98%, demonstrating utility of the technology for compound screening.
On the downside, application of SSM-based electrophysiology is limited to targets with an electrogenic net transport, assay setup is rather complex, and cost of ownership is significant. This may be an obstacle for implementing SURFE²R technology in-house. In response, Assay.Works entered into a partnership with Nanion, providing SURFE²R assays as a service. Backed by Nanions extensive expertise, we utilize our assay development and screening capabilities, including cell and membrane production, compound libraries, and sample management, to help clients realizing proof-of-concept, compound characterization, or profiling studies.

Download the full presentation as pdf file here.

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