• Nanion Technologies: Smart Tools for Ion Channel Research

    Nanion Technologies: Smart Tools for Ion Channel Research

  • SyncroPatch 384i: HTS Automated Patch Clamp

    SyncroPatch 384i: HTS Automated Patch Clamp

  • SURFE²R 96SE: Label-free HTS Transporter Screening

    SURFE²R 96SE: Label-free HTS Transporter Screening

  • Dynamic Clamp: Patchliner

    Dynamic Clamp: Patchliner

  • Bilayer recordings: Orbit product family

    Bilayer recordings: Orbit product family

  • CardioExcyte 96 SOL: Pacing Cardiomyocytes with Light

    CardioExcyte 96 SOL: Pacing Cardiomyocytes with Light

Our Product Portfolio

SyncroPatch 384

SyncroPatch 384

Patchliner

Patchliner

Port-a-Patch

Port-a-Patch

Port-a-Patch mini

Port-a-Patch mini

CardioExcyte 96

CardioExcyte 96

FLEXcyte 96

FLEXcyte 96

SURFE²R 96SE

SURFE²R 96SE

SURFE²R N1

SURFE²R N1

Orbit 16 TC

Orbit 16 TC

Orbit Mini

Orbit Mini

Vesicle Prep Pro

Vesicle Prep Pro

Buffer Solution

Buffer Solution

Bacterial Spheroplasts - "Preparation and recordings on the Port-a-Patch of native E. Coli spheroplasts"

icon pap   Port-a-Patch application note:   logo pdf   (0.5 MB) 

 Summary:

Bacterial membranes are not easy to patch clamp. Since bacterial ion channels are of increasing interest, we started to optimize the protocols for patch clamping bacterial spheroplasts with the Port-a-Patch. Bacterial spheroaplasts can be prepared up to a size of 5 µm. They consist of the inner bacterial membrane. This technique was first used for patch clamp experiments by Boris Matrinac in 1987 and let to the discovery of mechanosensitive channels in E. coli. Here we describe the preparation of Spheroplasts out of E. coli and show ion channel currents recorded with the Port-a-Patch.

Back to Overview

Nanion Corporate Blog

We use cookies on our website. Some of them are essential for the operation of the site, while others help us to improve this site and the user experience (tracking cookies). You can decide for yourself whether you want to allow cookies or not. Please note that if you reject them, you may not be able to use all the functionalities of the site.