13.10.2020 | Webinar: Impedance measurements and Calcium imaging to investigate the role of store operated Ca2+ entry in GPCR-mediated endothelial barrier regulation
CardioExcyte 96 Webinar
Date: October 13. 2020
Dr. Judith Stolwijk (University Hospital Regensburg; Germany)
This is an on-demand webinar from Nan]i[on and Friends 2020.
The endothelium of our blood vessels is under the control of various G protein-coupled receptors (GPCRs), regulating endothelial barrier function, vascular tone, angiogenesis and inflammation. Some of these GPCRs signal via Gq,11 protein, which activates Ca2+-release from the IP3-sensitive internal stores of the endoplasmic reticulum (ER). Calcium store depletion can subsequently activate so-called store-operated Ca2+ entry (SOCE) via the ER-resident Ca2+-sensor stromal-interacting molecule 1 (STIM1) and Orai1 Ca2+ entry channels.
We used Impedance measurements and ratiometric Calcium imaging to investigate the role of calcium entry via Orai1 channels downstream of barrier-regulating GPCRs in response to their agonists Thrombin, Histamine and Sphingosine-1-Phosphate (S1P) in human endothelial cells.