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2020 - Electrophysiology on Channel-Forming Proteins in Artificial Lipid Bilayers

Icon Orbit  Orbit 16 and Icon Orbit Mini Orbit mini Chapter in Patch Clamp Electrophysiology (2020)

Authors:
Zaitseva E., Obergrussberger A., Weichbrodt C., Boukhet M., Bernhard F., Hein C., Baaken G., Fertig N., Behrends J.C.

Book:
Patch Clamp Electrophysiology (2020) doi: 10.1007/978-1-0716-0818-0_4

Chapter 4:
Electrophysiology on Channel-Forming Proteins in Artificial Lipid Bilayers: Next-Generation Instrumentation for Multiple Recordings in Parallel


Abstract:

Artificial lipid bilayers have been used for several decades to study channel-forming pores and ion channels in membranes. Until recently, the classical two-chamber setups have been primarily used for studying the biophysical properties of pore forming proteins. Within the last 10 years, instruments for automated lipid bilayer measurements have been developed and are now commercially available. This chapter focuses on protein purification and reconstitution of channel-forming proteins into lipid bilayers using a classic setup and on the commercially available systems, the Orbit mini and Orbit 16.


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