Endoplasmatic Reticulum Membrane Protein Translocator (Sec61) - Single Channel recordings
Port-a-Patch and
Vesicle Prep Pro data and applications:
Data were kindly provided by A. Cavalié, M. Jung, R. Zimmermann, Universität des Saarlandes, Homburg, Germany.
Microsomes were fused with GUVs within the VesiclePrepPro. The resulted Proteoliposomes formed a bilayer onto the NPC-1 Chip of the Port-a-Patch. Nice single channel openings could be recorded in symmetrical KCl solution applying a ramp from -110 mV to +100 mV for 400 ms.