• Nanion Technologies: Smart Tools for Ion Channel Research

    Nanion Technologies: Smart Tools for Ion Channel Research

  • SyncroPatch 384i: HTS Automated Patch Clamp

    SyncroPatch 384i: HTS Automated Patch Clamp

  • SURFE²R 96SE: Label-free HTS Transporter Screening

    SURFE²R 96SE: Label-free HTS Transporter Screening

  • Dynamic Clamp: Patchliner

    Dynamic Clamp: Patchliner

  • Bilayer recordings: Orbit product family

    Bilayer recordings: Orbit product family

  • CardioExcyte 96 SOL: Pacing Cardiomyocytes with Light

    CardioExcyte 96 SOL: Pacing Cardiomyocytes with Light

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SyncroPatch 384

SyncroPatch 384

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CardioExcyte 96

CardioExcyte 96

FLEXcyte 96

FLEXcyte 96

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Orbit 16 TC

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Vesicle Prep Pro

Buffer Solution

Buffer Solution

FLEXcyte 96 - Contractility under physiological conditions


The FLEXcyte 96 brings in vitro contraction force measurements of cardiac cells to a new dimension. By using a well-defined, flexible substrate for cardiac cells, the system leverages maturity and mature responses of stem cell-derived cardiomyocytes to pharmacological compounds in a higher throughput format. In the 96-well plate, the cells adhere as monolayers on the flexible substrates and the contractility of the synchronized cell layers are recorded. This unique technology allows for recordings in a native-like environment close to mechanical conditions of the heart. The FLEXcyte 96 is a turn-key system for structural and functional contraction force recordings in cardiotoxicity, safety and efficacy screening and basic research.

The FLEXcyte 96 comes as an add-on for the CardioExcyte 96. With less than 10 μm in thickness and sophisticated surface modification, the polydimethylsiloxane (PDMS) membranes of the FLEXcyte 96 disposable plates offer physiological elasticity of native human heart tissue and strong mechanical support. While being deflected by the weight of the culture medium, rhythmic contraction of the cardiomyocytes lift the membranes in the 96-well upwards. By measuring the changes in deflection, the mechanical stress can be calculated.

The system has been validated with stem cell-derived cardiomyocytes from several providers (Ncardia, FUJIFILM Cellular Dynamics, Inc. (FCDI), Axol and NEXEL). Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC- CMs) display positive inotropic responses, no additional stimulation of “cell maturation” is necessary. Cardiac contraction force can also be measured on patient-derived cells.
No subtleties of cytotoxic responses are missed, measuring contractile forces on heart muscle cells for safety, efficacy and toxicity investigations in both chronic and acute time frames are performed reliably. The FLEXcyte 96 is a fully automated device, recording from 96 wells in parallel.

The benefits the FLEXcyte 96 offers include:

  • True contractility
  • Supports mature cell behavior
  • Physiological mechanical conditions
  • Higher throughput, 96-wells in parallel
  • Acute and chronic assessment of cell activity and toxicity
  • Optical pacing options

The CardioExcyte 96/ FLEXcyte 96 package includes an Incubation System which makes the system independent of an incubator. The system is placed on the bench top with maintained control over temperature, gas mix and humidity.
Efficiency and ease-of-use are facilitated by an outstanding software package for data handling and export - an essential part of the CardioExcyte 96/ FLEXcyte 96 system.

For detailed information:

Add-Ons and Features

CardioExcyte 96/ FLEXcyte 96 Platform

CardioExcyte Integra 1

The CardioExcyte 96/ FLEXcyte 96 package contains an Integra Viaflo Assist that provides automated multichannel pipetting for reproducible 96 well plate handling of cells and compounds.


FLEXcyte 96 Incubation System

FLEXcyte incubation system

The FLEXcyte 96 package includes an Incubation System. This means that the measurements are performed outside the cell culture incubator and the complete setup fits on a lab benchtop. The FLEXcyte 96 Incubation System controls temperature, gas mix and humidity. It is suitable for various experimental conditions (e.g. hypoxia).


FLEXcyte 96 SOL (Add-On)

Optical stimulation and investigation of contraction force in parallel:

CardioExcyte Sol 1

The stimulating optical lid, CardioExcyte 96/ FLEXcyte SOL, uses LEDs for spatially uniform stimulation of cells transfected with light-gated ion channels such as Channelrhodopsin2 (ChR2). After transfection of iPS cardiomyocytes with ChR2 the cells can be stimulated by blue light. Each light impulse induces a depolarization of the cells and thus an action potential is triggered. The advantages of optical stimulation over electrical stimulation include the highly precise timing, all cells of the beating network are stimulated exactly at the time of the light stimulus. In contrast, electrical stimulation propagates from the electrode across the well and thus cells are stimulated progressively. A mean beat calculation of precisely timed beats enables in-depth compound analysis and concentration response dependencies to be obtained. This is one of the key software capabilities of the CardioExcyte Control/ DataControl software package that is provided with each CardioExcyte 96/ FLEXcyte 96 system.


Software

 

CardioExcyte 96/ FLEXcyte 96 Software package

FLEXcyte 96 Analysis New

An adaptive signal detection algorithm extracts the positions and values of beating events. Besides beat intervals, amplitudes, rising and falling time, Pulse widths are detected. Furthermore, the area under the beat and arrhythmia (yellow) are identified and quantified as well.

The CardioExcyte/ FLEXcyte Control software supports online analysis of contractility parameters. The unique Mean Beat Function automatically visualizes the average of beat traces from one well, enveloped by the standard deviation, indicating the consistency of the beat pattern within the recorded well, i.e. if the cellular network is synchronously beating or not. The control software is intuitive and easy-to-use, at the same time very powerful in terms of experimental setup and online visualization of the recorded data.

The DataControl 96 software package is an independent software package to load and analyze data recorded on the CardioExcyte 96 or FLEXcyte 96. The analysis template can be saved and reloaded. Customized export formats of analysis results are integrated.

Consumables

 

FLX-96

FLEXcyte plate principle

The FLX-96 FLEXcyte membrane plates come in a standard 96-well format, allowing for automated liquid handling and easy integration into your lab procedures. After 7 days of culture, the cells have formed a biohybrid material with the membranes and are ready for assay. For further maturation in the physiological environment provided by the FLEXcyte 96 plates, the cells can be cultured for up to 4 weeks. The FLX-96 plates are a proprietary innovative product by innoVitro, developed for the FLEXcyte 96. It is produced and quality-assured in-house at innoVitro headquarters and shipped to our international customers.
With less than 10 μm in thickness and sophisticated surface modification, the polydimethylsiloxane (PDMS) membranes of the FLEXcyte 96 plates combine both physiological elasticity of native human heart tissue and strong mechanical support.


Description and Material

• Standard 96-well plate format
• Working volume: 200 µl (surface 34 mm2)
• Substrate: <10 µM thick PDMS (polydimethylsiloxane) flexible membrane
• Supports mature cell behavior


Available plate type:

"FLX-96, FLEXcyte 96 Membrane Plates - standard": Type standard

Watch the video on FLX-96 Plate Handling


Icon CE   CardioExcyte 96 and   Icon FLEX   FLEXcyte 96 product flyer on plates available  logo pdf   (0.90 MB) 

Testimonials & Case Studies

Data and Applications

Webinars and Movies

Downloads:

Application Notes

Product Sheets

Publications

Posters

Presentations

 

 

 

 

 

 

 

 

 

 

 

 

 

FLEXcyte 96 - White paper Download

 


FLEXcyte 96 and CiPA:

White Paper FLEXcyteThe aim of continued global efforts is to contribute towards the development of next generation assays reducing false positive and false negative results within drug development.

One goal of the FLEXcyte 96 assay is to be more predictive and biologically relevant compared with the technologies/approaches that it putatively replaces in early drug discovery. Furthermore, investigating contractility while providing a cost-efficient and reliable yet easy-to-operate system with a higher throughput using standard 96-well dimensions is unmet until today.

In line with the ongoing efforts of the stem cell working group of the CiPA initiative, the data output, physiological relevance of endpoints within the aim of investigating contractility in a wholistic way (electrophysiology, force and calcium transient measurements), the FLEXcyte 96 will make a precious contribution.


Read the publication here and download the full case study here:

Icon N1   SURFE²R Technology Customer Case Study (2020)   logo pdf   (1.3 MB)
Nature Scientific Reports: Characterization of the Na+/H+ exchanger NhaB on the SURFE²R N1


Co-Autor Dr. Miyer Fabián Patiño-Ruiz (University of Groningen, NL) comments on innovative SSM-based electrophysiology :

...a suitable technique to study bacterial ion-translocating membrane- systems which are otherwise difficult to approach using conventional electrophysiological techniques, like patch clamp.


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