• Nanion Technologies: Smart Tools for Ion Channel Research

    Nanion Technologies: Smart Tools for Ion Channel Research

  • SyncroPatch 384i: HTS Automated Patch Clamp

    SyncroPatch 384i: HTS Automated Patch Clamp

  • SURFE²R 96SE: Label-free HTS Transporter Screening

    SURFE²R 96SE: Label-free HTS Transporter Screening

  • Dynamic Clamp: Patchliner

    Dynamic Clamp: Patchliner

  • Bilayer recordings: Orbit product family

    Bilayer recordings: Orbit product family

  • CardioExcyte 96 SOL: Pacing Cardiomyocytes with Light

    CardioExcyte 96 SOL: Pacing Cardiomyocytes with Light

Our Product Portfolio

SyncroPatch 384

SyncroPatch 384

Patchliner

Patchliner

Port-a-Patch

Port-a-Patch

Port-a-Patch mini

Port-a-Patch mini

CardioExcyte 96

CardioExcyte 96

FLEXcyte 96

FLEXcyte 96

SURFE²R 96SE

SURFE²R 96SE

SURFE²R N1

SURFE²R N1

Orbit 16 TC

Orbit 16 TC

Orbit Mini

Orbit Mini

Vesicle Prep Pro

Vesicle Prep Pro

Buffer Solution

Buffer Solution

2021 User Meeting

210720 Welcome Banner UM 2021

2021 User Meeting - Munich
Register for the event here!


Confirmed Speakers (so far):

Rajnish Ranjan
EPFL - Blue Brain project

Dr. rer. nat. Wener Li
TU Dresden
Presenting: Blebbistatin protects iPSC-CM from hypercontraction and facilitates automated patch-clamp based electrophysiological study

Matthias Vömel (can only present on the 29th)
Bayer

David Adams
Illawarra Health & Medical Research Institute (IHMRI), University of Wollongong
Title: 

Fred Becq
University of Poitiers

Niels Voigt/Will Siebert
University of Göttingen

Chan Cao
École Polytechnique Fédérale de Lausanne

John Kasianowicz
Freiburg Institute of Advanced Studies

Tobias Ensslen 
Universiity of Freiburg 

Laurie A. Boyer

Massachusetts Institute of Technology


Transporter Webinar Series Talk - was already scheduled for this date: 10AM EST, 4PM CEST (this will be moved to another date)

Click Here to View the Complete Virtual Week Meeting Agenda(to be updated!) 

Our Collaborators 


Logo Columbi University Dept Psychiatry  logo universitaetsklinikum regensburg de   01 BRAIN Logo schwarz auf weiss CMYK 300dpi

logo innoVitro   Logo Metrion      Assay Works logo   sb logo updated 2020      fujifilm cdi
logo ionera 
       Logo accellerate   upcyte logo ratina    Evotec logo

Do you want to learn how to patch clamp in the space of one morning? Do you want to record ion channel activity from multiple cells simultaneously? Then you've come to the right place!

Free eBook: Patch Clamp Made Easy - a comprehensive guide for beginners

Automated Patch Clamp Technology

SyncroPatch 384 - For all your patch clamp needs

The SyncroPatch 384 is more than a high throughput screening system and can be implemented into all your electrophysiology projects, whatever your throughput needs. The 32-well mode of operation is ideal for smaller screening projects and academic research and takes full advantage of the economical price of the NPC-384 chip. Choose the number of parallel recordings that you need in multiples of 32, you can use the remaining wells over several days. Alternatively, you can use the SyncroPatch 384 in unattended mode for fully automated experiments for over 8 hours.

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Product Sheet

Interactive Brochure

High Throughput Pharmacology of NaV1.5 Channels on Nanion's SyncroPatch 384PE

Increase throughput by recording in unattended mode on the SyncroPatch 384

NaV1.5-ΔKPQ late INa current properties and pharmacology on the SyncroPatch 384i

 


Patchliner - Versatile and sophisticated

The Patchliner is a fully automated planar patch clamp instrument recording from up to 8 cells simultaneously. With its vast experimental freedom and gigaseal data quality, the Patchliner is one of the most versatile patch clamp instruments on the market.

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Product Sheet

Application Note - INa-Late recorded from CHO cells and hiPSC-CMs on Nanion´s Patchliner

Application Note - Pharmacology of hNaV1.5 recorded on Nanion's Patchliner

Application Note - Heat Activation of TRPV1

Publication - Automated Dynamic Clamp for Simulation of IK1 in Human Induced Pluripotent Stem Cell–Derived Cardiomyocytes in Real Time Using Patchliner Dynamite8


Port-a-Patch & Port-a-Patch mini - World's smallest patch clamp rig

The Port-a-Patch is a miniaturized patch clamp system supporting giga-seal recordings from one cell at a time. It offers fast and easy access to high quality patch clamp data with only minimal training. Not only a powerful research tool but also ideal for educational purposes and quick tests of cells and ion channels.

papmini300x300

Product Sheet  (Port-a-Patch)

Product Sheet (Port-a-Patch mini)

Product Video (Port-a-Patch mini)

 

Cell Monitoring Systems

CardioExcyte 96 – Combined contractility, electrophysiology and cell viability

The CardioExcyte 96 is a hybrid system recording both contractility and electrophysiology of intact cardiomyocyte networks for acute or chronic effects. In addition, the base impedance is continuously and automatically monitored for cytotoxic responses in contractile cardiac cells as well as non-contractile cell types such as hepatocyte-like cells or cancer cells.

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Product Sheet

Application Areas Infographic

The Cell Monitoring brochure


FLEXcyte 96 - Contractility under physiological conditions

The FLEXcyte 96 is an add-on for the CardioExcyte 96 which uses a unique flexible substrate allowing recordings in an in vivo-like environment to be performed which are close to the mechanical conditions of the heart and which greatly enhance the evaluation of drug candidates in cardiac safety, tox and efficacy studies.

FE300x300

Product Sheet

EVIDENCE Project Webinar

210907 EVIDENCE Webinar 1426x269 Leaderboard background Lars Nicoletta

Register Here - Join Live or Access On-Demand!

Time: 4PM CEST, 10AM EDT (Join Live or Access On-Demand)

Nicoletta Murciano
(PhD Candidate - Nanion Technologies)

Title: 
Automated patch clamp assay development for the study of red blood cells (RBCs) in health and disease

Abstract: 

Calcium (Ca2+) is a universal signalling molecule and is critically important in regulating many physiological functions and survival of RBCs. Amongst others, intracellular Ca2+ controls cell volume and deformability. This process plays a substantial role in RBCs since their volume needs to adapt when passing blood vessel constrictions during the flow. Excessive Ca2+ uptake also leads to accelerated cell clearance causing anaemia.

Therefore, studying Ca2+ regulation is crucial to understand RBC diseases. Piezo1, KCa3.1 (Gardos channel) and NMDA receptors are three channels present in the RBC membrane and critical for Ca2+ regulation.

We developed functional assays to measure these channels in healthy and diseased RBCs populations using electrophysiological tools, contributing to the characterization of RBC diseases.

Dr. Lars Kaestner
(Professor - Saarland University)


Title: 
Introduction to EVIDENCE prognostic, diagnostic, and therapeutic approaches on RBC function in health and disease

Abstract:

An introduction and overview to the EVIDENCE project.

 

Bilayer Webinar - RyR Receptor

210629 June Webinar 1426x26 Leaderboard background Filip RyR Webinar

Register Here - Join Live or Access On-Demand!

Time: 8AM PDT, 11AM EDT, 5PM CEST (Join Live or Access On-Demand)

Dr. Filip Van Petegem  (University of British Columbia)
(Professor, Biochemistry and Molecular Biology - University of British Columbia)

Title: 
Channelopathies resolved via cryo-EM and X-ray crystallography: an investigation of muscle excitation-contraction coupling

Abstract: 

Muscle contraction requires a tight communication between Ca2+-permeable ion channels located in the plasma membrane (L-type calcium channels, CaVs ) and in the Sarcoplasmic Reticulum (Ryanodine Receptors, RyRs).  In cardiac muscle, Ca2+ entering through CaV1.2 can stimulate the nearby RyR2 isoform, through a process of Ca2+-induced Ca2+ release.  In skeletal muscle, however, the coupling is thought to occur mechanically, with voltage-dependent conformational changes in CaV1.1 being transmitted to RyR1, either through direct or indirect interactions.  Mutations in RyR1 (as well as CaV1.1) can give rise to malignant hyperthermia (MH), a potentially lethal condition typically triggered by volatile anaesthetics. Other mutations cause central core disease (CCD), whereas some are linked to both MH and CCD.  Similarly, mutations in RyR2 are linked to a form of stress-induced cardiac arrhythmia, known as CPVT (catecholaminergic polymorphic ventricular tachycardia).  Our lab has been solving crystal structures of isolated RyR domains in WT and disease mutant forms, which show that many mutations cause conformational changes. More recently, we solved cryo-EM structures of RyR1 carrying the founding mutation (R615C) that links RyR1 to malignant hyperthermia. The structures show how many mutations can destabilize the closed state, leading to facilitated channel opening.

In addition, RyRs are heavily regulated by kinases (PKA, CaMKII) that can also facilitate channel opening. Excessive phosphorylation of RyRs has been linked to a range of acquired disorders, including atrial fibrillation and arrhythmias during heart failure.  We elucidated how PKA recognizes the cardiac RyR2 via an unusual interface and show that phosphorylation may induce the formation of extra secondary structure elements.

Finally, I will discuss our efforts to understand the mechanical coupling between CaV1.1 and RyR1 in skeletal muscle. STAC3 is a small protein recently shown to be essential for the coupling. We found this protein to interact with a cytosolic loop of CaV1.1 and found that several mutations in STAC3, linked to myopathy, directly affect this interaction and the mechanical coupling.

Dr. Conrad Weichbrodt 
(Product Manager of the Orbit product family - Nanion Technologies)



Title: 
Introduction to experiments on lipid bilayers 

Abstract:

Conrad will introduce Nanion’s Orbit devices for experiments on lipid bilayers and briefly explain how the micro electrode cavity array (MECA) consumables can support even unexperienced operators in generating artificial lipid bilayers and performing successive electrophysiological experiments.

 

Proposed 2021 Hybrid User Meeting (2-day In-house Event)

210616 pre registration proposed UM 2021 survey 1426x269 Leaderboard background

Register Here - Request to join In-House or Access with Remote / On-Demand!

When: 29-30 September, 2021
Where: Nanion Technologies HQ (Munich, Germany)


The two-day event will be a mixture of Live Product Demonstrations, User Talks and Networking 

Additional details to be announced shortly

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