The Port-a-Patch is a miniaturized patch clamp system supporting giga-seal recordings from one cell at a time. It offers fast and easy access to high quality patch clamp data with only minimal training. Not only a powerful research tool but also ideal for educational purposes and quick tests of cells and ion channels. The benefits the Port-a-Patch offers include:
- External & internal perfusion
- Temperature control
- Voltage & current clamp
- Whole-cell & perforated patch clamp
- Chips produced in-house, various resistances available (customizable on request)
- Rseries compensation
- Unlimited compound applications
- Voltage and ligand-gated ion channels
- High success rates with cell lines; use of primary cells and stem cells also possible
- Easy to use, minimal training required
- Adjustment of parameters during experiments for fast and efficient assay development
Using the Port-a-Patch is straightforward and easy – the user simply adds solutions and cells onto the disposable recording chip, where a cell is automatically captured and sealed by suction using a computer controlled pump. A microscope or micro-manipulator are not required.
Add-Ons such as the Internal and External Perfusion and Temperature Control, as well as the ability to conduct current clamp and bilayer experiments, broaden the range of possible experiments for scientific research immensely.
The Port-a-Patch system is comprised of the Port-a-Patch recording unit, a Suction Control unit, a HEKA amplifier and a computer. If an amplifier is already available, this can be integrated with the Port-a-Patch system in most cases. Further add-ons are available for specific assays as e.g. temperature control, microscope slides or automated perfusion systems.
For detailed information:
Add-Ons and Features
Temperature controlled external perfusion
The External Perfusion System for the Port-a-Patch is comprised of a computer controlled magnetic pinch valve panel and a dedicated temperature controllable flow chamber (TC Flow Chamber). Up to eight different solutions can be handled by the perfusion panel. Solutions can either be switched manually or via the software. The latter allows synchronization of data acquisition with solution exchange, as well as tagging of the collected traces with relevant information such as compound and concentration applied.
The TC Flow Chamber which is provided with the Perfusion Panel has a solution exchange time of <150 ms making it ideal for studies of both ligand and voltage gated channels.The Temperature Control feature of the TC Flow Chamber allows experiments at hot AND cold temperatures or application of short temperature pulses. Two components are used to heat or cool the solutions: a cannula for cooling/heating the solution during continuous perfusion, and a Peltier element integrated in the Faraday cage. This results in stable temperature maintenance whether or not the cell is perfused. A temperature sensor will provide real-time information of the bath solution and be monitored in PatchControl which can adjust the temperature with the click of a button.
Internal Perfusion System
Nanion’s planar patch clamp chips offer the unique possibility to perfuse the intracellular side of the membrane. This aspect is exploited with the Internal Perfusion System for the Port-a-Patch. Up to eight different solutions can be perfused inside the cell, allowing dose response analysis of compounds acting on the cytosolic portion of the ion channel. Second messengers and metabolites can also be added internally to investigate how ion channel function is affected. Solutions are exchanged within seconds, and the ion channel modulation can be continuously monitored during the solution exchange.
The Internal Perfusion System is an Add-On for the Port- a-Patch. Combined with Nanion’s Perfusion System it can be operated either manually, or fully computer controlled. In this way, data acquisition can be synchronized with solution exchange and traces automatically tagged with the compound information.
Port-a-Patch Microscope Slide
Nanion offers the Port-a-Patch Microscope Slide developed for the simultaneous visualization and patch clamp recording of the cell. Fluorescence measurements using an upright microscope can therefore be combined with current recordings.
Suction Control Pro
The Suction Control Pro contains an interface for communication with the analog in- and outputs of the patch clamp amplifier. Via this communication route, pressure application can be synchronized with voltage protocols and they can be simultaneously recorded.
The Suction Control Pro is ideally suited for studies of mechanosensitive ion channels and for studying the effect of pressure on artificial and cellular membranes. The add-on is available in conjunction with the Port-a-Patch. It is compatible with all existing Port-a-Patch systems.
The Port-a-Patch SOL
The Port-a-Patch SOL enables optical stimulation during recordings on the Port-a-Patch by covering it with a dedicated lid to which up to three light sources can be connected.
The Port-a-Patch SOL is available as a kit or as an Add-On.
The Port-a-Patch SOL Kit consists of an optical lid and one high-power LED light source. The light source can either controlled manually or by the amplifier software.
The Add-On does not include a light source, so you are free to use light sources you already have.
PatchContol: the intuitive Software of the Port-a-Patch
PatchControl is a software programme written with the user in mind. Pre-written protocols can be loaded and altered, allowing the entire procedure for cell capture, sealing and obtaining the whole cell configuration to be completely automated. However, because we know that sometimes you just want to control things yourself, the protocols can be interrupted at any time and the suction controlled manually.
Through innovative communication with electrophysiology software, PatchControl can read important parameters such as membrane resistance, whole cell capacitance and series resistance and moves through the pre-programmed steps accordingly. Parameters such as suction strength and duration, voltage and membrane criteria can be set and altered to obtain the optimum parameters for cells routinely used in your lab. Not only that, complex macros can be easily implemented, allowing everything from simple changes in gain to complex perfusion routines to be programmed and automatically performed.
The PatchControl software is there to make your patch clamp life easier, from automated cell capture to automated pressure control, the PatchControl software can do it all!
The NPC-1 chip is a proprietary innovative product by Nanion Technologies, developed for the Port-a-Patch. It is produced and quality-assured in-house at Nanion headquarters and shipped from Munich to our international customers. Different types of NPC-1 chips are available which should be chosen depending on cell size and application.
MaterialThe core structure of the NPC-1 chip is a planar borosilicate glass slide with its micron-sized patch aperture. This structure is incorporated into a screw cap for easy handling and minimal cycle times between experiments. Additionally, the unique design of the NPC-1 chip gives similar access to the external and internal solution. Continuous perfusion of the internal solution has finally become possible.
Available chip types
- "NPC-1, 1-2 MOhm": (Order # 061101)
- "NPC-1, 2-3.5 MOhm": (Order # 061102)
- "NPC-1, 3-5 MOhm": (Order # 061103)
- "NPC-1, 5-10 MOhm": (Order # 061104)
- "NPC-1, 8-12 MOhm": (Order # 061105)
- "NPC-1, 10-15 MOhm": (Order # 061106)
Other sizes are available upon request!
Buffers and Solutions for the Port-a-Patch
Reliable buffer solutions are critical for any electrophysiological application. Our goal, therefore, is to provide high-class recording solutions that leave you in no doubt about quality and stability. Our quality assurance includes chemical tests as well as tests on our patch clamp systems of each lot. Our buffers are shipped with the corresponding "Certificates of Analysis" and "Material Safety Data Sheets" (MSDS).
Available buffers and solutions
- "External Standard", 500 mL: (Order # 08 3001)
- "External Standard Ca 10", 500 mL: (Order # 08 3012)
- "External NMDG 60", 500 mL: (Order # 08 3004)
- "External NMDG 60 Ca 10", 500 mL: (Order # 08 3011)
- "Internal CsF 110", 500 mL: (Order # 08 3008)
- "Internal KF 110", 500 mL: (Order # 08 3007)
- "Reagent Kit Port-a-Patch Standard", Cryo box with 81 vials, incuding 27x External standard, 18x External Ca 35, 18x Internal KF 60, 18x Internal CsF 60 (Order # 08 1001)
Data and Applications
Testimonials & Case Studies