The Patchliner is a fully automated planar patch clamp instrument recording from up to 8 cells simultaneously. With its vast experimental freedom and gigaseal data quality, the Patchliner is one of the most versatile patch clamp instruments on the market. The benefits the Patchliner offers include:
- External & internal exchange
- Unlimited compound applications due to continuous waste removal
- Temperature control (see Applications)
- Cooled CellHotel
- Voltage & current clamp + dynamic clamp
- Available as Patchliner Quattro or Octo – 4 or 8 amplifier channels
- Single- & multihole chips, produced in-house
- Rseries compensation
- Voltage and ligand-gated ion channels
- Temperature-activated channels
- Recordings at physiological temperature
- Whole-cell & perforated patch clamp
- Cell lines, primary cells and stem cells
- Part of the CiPA validation study
- Minimized cell consumption
- Easy & customizable analysis tools
The Patchliner is versatile yet robust, ideal for basic research of ion channel biophysics and mechanisms of action, and sophisticated assays including heat activation of TRP channels, activation of Ca2+-activated channels by internal exchange and fast external solution exchange with minimal exposure for ligand-gated ion channels such as nAChα7. The Patchliner is also an excellent tool for routine assays such as safety screening of hERG or other cardiac ion channels in line with the CiPA initiative.
Since its introduction in 2006, over 100 instruments have been installed worldwide, in academic labs (46%), pharmaceutical companies (34%) and CROs (20%). The Patchliner is appreciated because of routine high success rates (>80% gigaseals), optimized assays including minimized cell consumption and proven use of primary cells and stem cells.
Our dedicated team of electrophysiologists and engineers are committed to continuous in-house assay development, software and hardware advancements, ensuring fast and custom-tailored solutions for your assay demands.
For detailed information:
Add-ons and Features
The Dynamite8 is a fully automated dynamic clamp add-on integrating seamlessly into an 8-channel or 4-channel Patchliner. IK1 simulation and seal compensation are individually calculated for each cell, for up to 8 cells simultaneously. Applying our dynamic clamp method results in stable, negative resting membrane potentials and improved stability and shape of APs recorded from human induces pluripotent stem-cell-derived cardiomyocytes (hiPSC-CMs).
Stable and reliable AP recordings allow for the acquisition of full, cumulative dose-response curves, providing the basis for automated AP pharmacology recordings for cardiac safety and drug testing. No user intervention is required during experiments: all parameters are calculated and applied by the Dynamite8. If desired, all critical parameters are easily accessible for modification through the user.
The Patchliner CoolingPlate integrates into the Patchliner for temperature control of cells and solutions. Cells can be kept at a cooled temperature for increased seal resistance and whole cell stability, even hours after harvesting. Solutions can also be cooled to improve stability, e.g. ATP-containing internal solution. The CoolingPlate provides space for a CellHotel and 8 Eppendorf tubes. It is simple to install and easily controlled via PatchControl HT.
Patchliner Safety edition
The Patchliner Safety Edition is set up to readily run safety pharmacology protocols and analysis routines. It comes with pre-installed routines and SOPs for hERG, NaV1.5-peak, NaV1.5-late and CaV1.2 according to the CiPA guidelines. The training is focused on safety pharmacology experiments and of course adaptable to specific user requirements. The Patchliner Safety Edition includes temperature control for measurements at physiological temperature, the Patchliner CoolingPlate for viability of cells and stability of compounds, and the Dynamite8 for dynamic clamp action potential recordings of hiPSC-derived cardiomyocytes. In addition, we offer expert support from Nanion’s CiPA specialists.
PatchControl HT is the software for the Patchliner. The vast experimental freedom of the Patchliner platform is due to the flexibility of the software.
PatchControl HT is a graphical user interface allowing straightforward and easy programming of protocols either by using pre-programmed modules, or by setting up customized protocols based on the specific user requirements.
Because of its inherent logical programming capabilities, experimental redundancy is reduced to a minimum, thus maximizing the data throughput.
PatchControl HT not only allows the running of experiments from start to finish, it also supports changes on-the-fly. This latter feature speeds up the assay development process immensely. It also makes the Patchliner a valuable tool for research.
Data can easily be analyzed using Nanion's Data Analysis Package, a very efficient and convenient data analysis tool. With a few mouse clicks raw data is loaded, displayed, analyzed and pooled, reducing the effort to a minimum.
Display of raw current traces at this point allows judgement of the quality of the data recorded from each cell. Individual cells can be easily excluded from the data set and averages recalculated.
Current Voltage relationship plots as well as IC50 values are automatically calculated and displayed.
The NPC-16 chip is a proprietary innovative product by Nanion Technologies, developed for the Patchliner. It is produced and quality-assured in-house at Nanion headquarters and shipped from Munich to our international customers. Different types of NPC-16 chips are available which should be chosen depending on cell size and application.
The borosilicate glass slide with the patch aperture is encased in a cartridge forming the microfludic channels through which the solutions are delivered. The design of the chip allows as well an internal solution exchange during an experiment.
Each NPC-16 chip contains 16 recording chambers.
Depending on the Patchliner version (Quattro, Octo) 4 or 8 of these sites are used at a time. Three chips can be loaded onto the Patchliner. 48 recordings can be performed without user intervention.
Additionally, the number of exchanges of either the internal or the external solution is unlimited.
Available chip types
- "NPC-16, 1x, medium resistance": One hole well, 1.8 - 3 MOhm (Order # 071102)
- "NPC-16, 4x, medium resistance": Four hole wells, 1.8 - 3 MOhm (Order # 071401)
- "NPC-16, 1x, low resistance": One hole wells, 1.5 - 2 MOhm (Order # 071103)
- "NPC-16, 1x, high resistance": One hole wells, 3 - 5 MOhm (Order # 071101)
- "NPC-16, 1x, ultra-high resistance": One hole wells, 5 -8 MOhm (Order # 071104)
Other sizes are available upon request!
Buffers and Solutions for the Patchliner
Reliable buffer solutions are critical for any electrophysiological application. Our goal, therefore, is to provide high-class recording solutions that leave you in no doubt about quality and stability. Our quality assurance includes chemical tests as well as tests on our patch clamp systems of each lot. Our buffers are shipped with the corresponding "Certificates of Analysis" and "Material Safety Data Sheets" (MSDS).
Available buffers and solutions
- "External Standard", 500 mL: (Order # 08 3001)
- "External Standard Ca 10", 500 mL: (Order # 08 3012)
- "External NMDG 60", 500 mL: (Order # 08 3004)
- "External NMDG 60 Ca 10", 500 mL: (Order # 08 3011)
- "Internal CsF 110", 500 mL: (Order # 08 3008)
- "Internal KF 110", 500 mL: (Order # 08 3007)
Data and Applications
Case Studies and Testimonials
Webinars and Movies
Movies: Tutorials and Oral Presentations