• Our CiPA Instruments

    Patchliner & SyncroPatch 384PE (CiPA ion channel working group); CardioExcyte 96 (CiPA myocyte working group)

  • CiPA hERG Protocol

    This protocol was used for hERG studies on the Patchliner and SyncroPatch 384PE.

  • HTS CiPA hERG Assay

    Effects of Cisapride using the CiPA hERG protocol on the SyncroPatch 384PE

  • Myocyte & Ion Channel Effects

    Arrhythmic Field potentials in iPSC-derived Cardiomyocytes (CardioExcyte 96) and hERG current inhibition (SyncroPatch 384PE)

  • Gigaseal HTS patch clamp

    CiPA-specified cardiac ion channels recorded at high throughput

  • Gigaseal HTS patch clamp

    High throughput recordings of cardiac ion channels at physiological temperature

  • CardioExcyte 96 screening tool

    CardioExcyte 96 with integrated liquid handling for cardiac safety screening

2015 - High Throughput Automated Patch Clamp of Ion Channels Important in Cardiac Safety and Drug Discovery

icon sp96  SyncroPatch 384PE poster, Chantest Meeting 2015   logo pdf   (1.9 MB)

 Abstract:

One important part of the drug discovery process is the early assessment of a drug’s safety profile. The new paradigm in cardiac drug safety screening, the Comprehensive In-vitro Proarrhythmia Assay (CiPA) initiative, is being introduced to provide a more complete assessment of proarrythmic risk by evaluating and implementing currently available high throughput methods. An important part of this is an extension of the electrophysiological evaluation of ion channels beyond hERG to include other cardiac channels such as NaV1.5, CaV1.2, KVLQT1 (KV7.1) and Kir2.1. The ion channel CaV1.2, however, is not only found in cardiac tissue but also in the CNS and smooth muscle cells, amongst others. It is thought to play a role in CNS function, cardiac and smooth muscle contraction, neuroendocrine regulation and a multitude of other processes (Hofmann, Flockerzi, Kahl, & Wegener, 2014). Blockers of CaV1.2 are used for the treatment of hypertension and angina and pharmacology of two such compounds (verapamil and nifedipine) are shown here. In this study, HEK or CHO cells expressing the ion channels hERG, NaV1.5, CaV1.2, KVLQT1 (KV7.1), KV4.3 and Kir2.1 were recorded simultaneously on an automated patch clamp instrument recording from 384 cells in parallel. Additionally, CHO cells expressing CaV1.2 were recorded using multi-hole chips and pharmacology of verapamil and nifedipine will be shown.

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