• Alamethicin

    Alamethicin single channel conductances, measured on the Port-a-Patch. Recordings from a GUV prepared bilayer

Alamethicin

Family:
Non-ribosomally Synthesized Channels

Description:
Alamethicin is a channel-forming peptide antibiotic, produced by the fungus Trichoderma viride. It belongs to peptaibol peptides, which contain the non-proteinogenic amino acid residue Aib (2-aminoisobutyric acid).

Topology:
Chemically, the peptaibol is a peptide of 20 amino acids and forms a alpha-helical structure. In cell membranes, it forms voltage-dependent ion channels by aggregation of four to six molecules.

 

Data and Applications

Alamethicin - Parallel recordings

Alamethicin IoneraIcon Orbit   Orbit 16 data and applications: 
Data courtesy of Dr. Gerhard Baaken, University of Freiburg / Ionera.

The data image shows parallel recordings from reconstituted alamethicin channels. See also the paper: "Alamethicin Supramolecular Organization in Lipid Membranes from 19F Solid-State NMR", Salnikov et al. (2016) Biophysical Journal 111(11): 2450-2459.

Alamethicin - Bilayer Recordings

application alameticin 2icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro data and applications:
Data were kindly provided by M. Sondermann/Prof. Behrends, Univ. Freiburg.

Alamethicin is a channel forming peptide and, when patch clamped, reveals multiple non-equidistant conductance levels due to formation of alamethicin oligomers in the bilayer. Alamethicin single channel conductances. Recordings from a GUV prepared bilayer in 85 mM KCl at -140 mV.

Publications

2016 - Alamethicin Supramolecular Organization in Lipid Membranes from 19F Solid-State NMR

Icon Orbit  Orbit 16 publication in Biophysical Journal (2016)

Authors: 
Salnikov E.S., Raya J., De Zotti M., Zaitseva E., Peggion C., Ballano G., Toniolo C., Raap J., Bechinger B.

2012 - Natural and artificial ion channels for biosensing platforms

icon pap   Port-a-Patch,   icon pl   Patchliner,   icon sp96   SyncroPatch 96 ((a predecessor model of SyncroPatch 384PE) and   icon vpp   Vesicle Prep Pro publication in Analytical and Bioanalytical Chemistry (2012)

Authors: 
Steller L., Kreir M., Salzer R.

2008 - Planar microelectrode-cavity array for high-resolution and parallel electrical recording of membrane ionic currents

Icon Orbit  Orbit 16 publication in Lab on a Chip (2008)

Authors:
Baaken G., Sondermann M., Schlemmer C., Rühe J., Behrends J.C.

2006 - High-resolution electrophysiology on a chip: Transient dynamics of alamethicin channel formation

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Biochimica et Biophysica Acta - Biomembranes (2006)

Authors: 
Sondermann M., George M., Fertig N., Behrends J.C.

 

 

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