• Ammonia Transporter Amt

    SURFE²R N1 recordings of the ammonium transporter AmtB. AmtB was purified from E.Coli and incororated into liposomes. Mirandela et al, 2018.

AmtB - Substrate specificity

AmtB specificity 1

Icon N1 SURFE2R N1 data and applications:

Purified AmtB from E.Coli was incorporated into liposomes and used on the SURFE2R N1. AmtB substrate specificity. (A) Transient current measured after a 100 mM substrate jump. Ammonium (red), methylammonium (black), potassium (green) or sodium (purple). Potassium and sodium do not act as substrates for AmtB and methylammonium translocates but at a much reduced rate compared with ammonium. Insert: Normalised current after a 100 mM substrate jump. Ammonium (red), methylammonium (black).

Data from Mirandela et al, 2018

 

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