• Bacteriorhodopsin

    measured on the SURFE2R N1

BR – Action vs. Absorption Spectra on SURFE2R N1

BR Figure 2  wavelength

Icon N1   SURFE2R N1 data and applications:

Light induced currents have been measured using five LED sensor plates with different emission wavelengths. The wavelength dependent peak currents (action spectra) overlay with the absorption spectrum of BR

Author: Nanion Technologies, in cooperation with Dimitrios Fotiadis, University of Bern
Target (Synonyms): BR, Bacteriorhodopsin
Family / Type: Ion-translocating Microbial Rhodopsin Family (MRF, TCDB: 3.E.1)
Mode of transport: light-driven H+ pump
Organism: Halobacterium salinarum
Sample: Purple membrane preparation
Expression: Native
Platform: SURFE2R N1

Background and Assay Description:
The different LED sensor plates of SURFE2R SOL can be exchanged manually within seconds, allowing for subsequent light induced measurements using different wavelength on the same sensor. Here we have compared the current induced by royal blue (λmax = 450 ± 10 nm), blue (λmax = 470 ± 10 nm), green (λmax = 530 ± 10 nm), amber (λmax = 587 ± 2.5 nm) and red-orange (λmax = 615 ± 12.5 nm) LED plates at 50 mA operating power. The absorption spectra was recorded in continous mode using a standard photometer. To achieve a direct comparison, we normalized the peak currents and absorption spectrum to the values obtained for 530 nm. The overlay shows that the BR transport activity is a result of photon adsorption by retinal.

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