• CaV3.3

    Current traces measured on the SyncroPatch 384PE

    Prof. Jen Q. Pan (Broad Institute of MIT and Harvard), Webinar (2020)

CaV3.3 | voltage-dependent, T type, calcium channel subunit alpha-1I

Family:
Calcium channels

Subgroups:
L-Type (CaV1.1–CaV1.4), P/Q-Type (CaV2.1), N-Type (CaV2.2), R-Type (CaV2.3), T-Type (CaV3.1–CaV3.3)

Topology:
Six transmembrane alpha helices (S1–S6), total of four homologous domains make up the tetrameric alpha subunit structure

Assembling:
One large alpha subunit forms a functional channel, accessory subunits ( α1, α2δ, β1-4, and γ) are crucial for robust expression, they functionally modulate the alpha subunit

CaV3.3 Background Information


Overview:

Calcium channel, voltage-dependent, T type, alpha 1I subunit, also known as CACNA1I or CaV3.3, is a low voltage-activated channel with shows a moderate voltage-dependent inactivation. The channel generates low-threshold Ca2+ spikes leading to burst firing and low-frequency oscillatory discharge. Mutations of the CACNA1L gene are associated with epilepsy. CaV3.3 may be involved in sleep regulation.


Data Sheet:

Genes:
CACNA1I

Human Protein:
UniProt Q9P0X4

Tissue:
CNS ( highly expressed in thalamic reticular neurons) and PNS.

Function/ Application:
Low threshold Ca2+ spike that mediates burst firing of neurons.

Pathology:
Childhood Absence Epilepsy, Childhood Electroclinical Syndrome, Epilepsy

Interaction:
Interacts with CATSPER1 and CATSPER2, leading to suppress T-type calcium channel activity. Furthermore it interacts with CACNB1, CACNB2, CACNB3, CACNA1B, CACNA1D, CACNA1E.

Modulator:
Mibefradil, nickel, verapamil

Assays:
Patch Clamp: whole cell, room temperature

Particularities:
CaV channels often show a rundown phenomenon. Adequate intra- and extracellular solutions are essential for a good data quality.

Reviews and Links

Publications

2019 - Predicting Functional Effects of Missense Variants in Voltage-Gated Sodium and Calcium

icon sp96   SyncroPatch 384PE (a predecessor model of the SyncroPatch 384i) Pre-publication in bioRxiv Biology (2019)

Authors:
Heyne H.O., Baez-Nieto D., Iqbal S., Palmer D., Brunklaus A., Johannesen K.M., Lauxmann S., Lemke J.R., Møller R.S., Pérez-Palma E., Scholl U., Syrbe S., Lerche H., May P., Lal D., Campbell A.J., Pan J., Wang H.-R., Daly M.J.

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