ChR2
Family: Rhodopsins (TCDB: 3.E.1 - Ion-translocating Microbial Rhodopsin (MR) Family)
Subfamily: Channelrhodopsins (ChR)
Members: ChR1, ChR2 and several variants as well as designed mutations
Description: Channelrhodopsins (ChRs) are light-gated cation channels derived from algae. They are seven-transmembrane proteins containing the light-isomerizable chromophore retinal. ChRs serve as photoreceptors in microbial organisms and are a commonly used tool in optogenetics.
ChR2 Background Information
Overview:
ChR2 is
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Data and Applications
ChR2 - Variation of light intensity
SURFE2R N1 data and applications:
We observed saturation kinetics for the light-induced ChR2 activity with increasing LED intensities using a prototypical SURFE2R N1 add-on for activation by light.
ChR2 - Symmetry of transport
SURFE2R N1 data and applications:
Depending on the direction of the Na+ gradient, we observed positive or negative transient currents after ChR2 activation by light. A negative control without ChR2 shows no current signal upon light activation.
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ChR2 - Stability of Retinal binding
SURFE2R N1 data and applications:
We investigated the effects of retinal addition and removal on light-induced ChR2 activity using a prototypical SURFE2R N1 add-on for activation by light.
ChR2 - Membrane potential as driving force
SURFE2R N1 data and applications:
We investigated the effect of a membrane potential - generated by Na+/Ca2+ exchange activity of NCX1 - on the signal amplitude of light induced Na+ flux by ChR2.
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ChR2 - Exposure time and frequency of light
SURFE2R N1 data and applications:
We investigated the influence of the duration and frequency of the light pulses for ChR2 activation on the stability of the ChR2 signals over time.
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ChR2 - Cell-based assay optimization
SURFE2R N1 data and applications:
For assay optimization we checked the influence on cell suspension volume we have attached to one single sensor on the signal-to-noise ratio during the SURFE2R experiment.
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Cardiomyocytes - Optogenetics meets cardiac safety
CardioExcyte data and applications:
Cells were kindly provided by Axiogenesis.
The stimulating optical lid, CardioExcyte 96 SOL, uses LEDs for spatially uniform stimulation of cells transfected with light-gated ion channels such as Channelrhodopsin2 (ChR2).
Right graph: LPM – Light pulse per minute plotted against the recorded beat rate (average of 96 wells). ChR2 transfected Cor.4U cells are following the optical pace rate.
Cardiomyocytes - Myocyte phase II study: CiPA conform analysis and arrhythmia detection
CardioExcyte data and applications:
Cells were kindly provided by Axiogenesis.
Nanion developed a CiPA conform analysis for the Myocyte phase II study. The feature comes along is included in our CiPA analysis routine. Automated arrhythmia detection is just one highlight out of many when it comes to the CardioExcyte 96 software.
Cardiomyocytes - Channelrhodopsin 2 (ChR2) transfected Cor.4U cells and optical pacing
CardioExcyte data and applications:
Cells were kindly provided by Axiogenesis.
ChR2 transfected Cor.4U cells are following the optical pace rate.
Raw data traces upon a 1 Hz, 1.5 Hz, 2 Hz., 2.5 Hz and 3 Hz stimulation rate, extracellular field potentials (top) and impedance (bottom).
Publications
2020 - Wireless Charging Electrochemiluminescence System for Ionic Channel Manipulation in Living Cells
Port-a-Patch publication in ACS Applied Materials & Interfaces (2020)
Authors:
Peng K., Liu S., Lv F., Fu X., Hussain S., Zhao H., Liu L., Wang S.
2018 - An update on the advancing high-throughput screening techniques for patch clamp-based ion channel screens: implications for drug discovery
SyncroPatch 384/768PE (a predecessor model of SyncroPatch 384/768i) and 
Patchliner publication in Expert Opinion on Drug Discovery
Authors:
Obergrussberger A., Goetze T.A., Brinkwirth N., Becker N., Friis S., Rapedius M., Haarmann C., Rinke-Weiß I., Stölzle-Feix S., Brüggemann A., George M., Fertig N.
2017 - Frequency-Dependent Multi-Well Cardiotoxicity Screening Enabled by Optogenetic Stimulation
CardioExcyte 96 publication in International Journal of Molecular Sciences
Authors:
Rehnelt S., Malan D., Juhasz K., Wolters B., Doerr L., Beckler M., Kettenhofen R., Bohlen H., Bruegmann T., Sasse P.