ChR2 - Cell-based assay optimization
For assay optimization we checked the influence on cell suspension volume we have attached to one single sensor on the signal-to-noise ratio during the SURFE2R experiment.
Background and Assay Description:
We have performed an assay on ChR2 activity using whole cells on the SURFE2R N1 sensor chips. Cells were detached directly before use and suspended in assay buffer. We used different volumes of cell suspension during sensor preparation and found that 80 μl of cell suspension yield the maximum signal-to-noise during the SURFE2R experiment. With lower volume the cells are more stably adhered to the sensors, but the sensor surface is not fully covered with cells, while at higher volume the cells are not stably attached.
Author: Nanion Technologies and Axxam S.p.a.
Target: T159C ChR2
Family / Type: Ion-translocating Microbial Rhodopsin Family (MRF, TCDB: 3.E.1)
Mode of transport: Light-gated Na+ flux
Organism: Chlamydomonas reinhardtii
Sample: Whole HEK293 cells
Expression: Recombinant expression in HEK293 cells
Platform: SURFE²R N1