Clc

Family:
Chloride Carrier/Channel Family

Subgrops:

Topology:

Function:

Data and Applications

Clc - Proteoliposomes with different lipid-to-protein ratios

Clc Figure 4   LPRsIcon N1   SURFE2R N1 data and applications:

We tested proteoliposomes with different densities of reconstituted Clc protein for their signal-to-noise ratios and compared the obtained signal amplitudes with a negative control: liposomes without reconstituted protein.

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Clc - Inward and outward directed chloride flux

Clc Figure 1   transient currents Influx vs EffluxIcon N1   SURFE2R N1 data and applications:

According to the transport direction of 2Cl-/1H+ exchange, we observed positive (Cl- efflux) or negative (Cl- influx) transient currents.



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Clc - Inhibition with OADS

Clc Figure 3   OADS inhibitionIcon N1   SURFE2R N1 data and applications:

We investigated the effect of the specific Clc inhibitor OADS on the transport activity of Clc and its reversibility.



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Clc - EC50 for inward and outward directed chloride flux

Clc Figure 2   EC50 Efflux vs InfluxIcon N1   SURFE2R N1 data and applications:

We determined EC50 and relative Vmax for Cl- in an Cl-/H+ exchange assay for both transport directions and found a slight asymmetry in transporter kinetics.

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Posters

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Publications

2019 - Structure of the human ClC-1 chloride channel

icon pap   Port-a-Patch,  icon vpp   Vesicle Prep Pro and   Icon Orbit Mini   Orbit mini publication in PLOS Biology (2019)

Authors:
Wang K., Preisler, SS, Zhang, L., Cui, Y., Missel, JW., Grønberg C., Gotfryd, K., Lindahl E., Andersson, M., Calloe, K., Egea P.F., Klaerke D.A., Pusch M., Pedersen P.A., Zhou, Z.H., Gourdon, P.

2013 - Functional characterization of a ClC transporter by solid-supported membrane electrophysiology

Icon N1   SURFE²R-technology (custom-built system) publication in Journal of General Physiology (2013)

Authors:
Garcia-Celma J., Szydelko A., Dutzler R.

2010 - The G215R Mutation in the Cl−/H+-Antiporter ClC-7 Found in ADO II Osteopetrosis Does Not Abolish Function but Causes a Severe Trafficking Defect

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in PLoS ONE (2010)

Authors:
Schulz P., Werner J., Stauber T., Henriksen K., Fendler K.

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