Clc
Family:
Chloride Carrier/Channel Family
Subgrops:
Topology:
Function:
Data and Applications
Clc - Proteoliposomes with different lipid-to-protein ratios
SURFE2R N1 data and applications:
We tested proteoliposomes with different densities of reconstituted Clc protein for their signal-to-noise ratios and compared the obtained signal amplitudes with a negative control: liposomes without reconstituted protein.
Clc - Inward and outward directed chloride flux
SURFE2R N1 data and applications:
According to the transport direction of 2Cl-/1H+ exchange, we observed positive (Cl- efflux) or negative (Cl- influx) transient currents.
Clc - Inhibition with OADS
SURFE2R N1 data and applications:
We investigated the effect of the specific Clc inhibitor OADS on the transport activity of Clc and its reversibility.
Clc - EC50 for inward and outward directed chloride flux
SURFE2R N1 data and applications:
We determined EC50 and relative Vmax for Cl- in an Cl-/H+ exchange assay for both transport directions and found a slight asymmetry in transporter kinetics.
Posters
Publications
2019 - Structure of the human ClC-1 chloride channel
Port-a-Patch, 
Vesicle Prep Pro and 
Orbit mini publication in PLOS Biology (2019)
Authors:
Wang K., Preisler, SS, Zhang, L., Cui, Y., Missel, JW., Grønberg C., Gotfryd, K., Lindahl E., Andersson, M., Calloe, K., Egea P.F., Klaerke D.A., Pusch M., Pedersen P.A., Zhou, Z.H., Gourdon, P.
2013 - Functional characterization of a ClC transporter by solid-supported membrane electrophysiology
SURFE²R-technology (custom-built system) publication in Journal of General Physiology (2013)
Authors:
Garcia-Celma J., Szydelko A., Dutzler R.
2010 - The G215R Mutation in the Cl−/H+-Antiporter ClC-7 Found in ADO II Osteopetrosis Does Not Abolish Function but Causes a Severe Trafficking Defect
SURFE²R ONE (a predecessor model of SURFE²R N1) publication in PLoS ONE (2010)
Authors:
Schulz P., Werner J., Stauber T., Henriksen K., Fendler K.