• ClC-1

    Tail currents of ClC-1 expressed in CHO cells inhibited by increasing concentration of 9-AC on the SyncroPatch 384PE

  • Ec-ClC

    Typical Ec-ClC current response on the SURFE2R N1 (left) when chloride is applied to the sensor and the corresponding concentration response curve for chloride (right).

ClC - Inward and outward directed chloride flux

Clc Figure 1   transient currents Influx vs EffluxIcon N1   SURFE2R N1 data and applications:

According to the transport direction of 2Cl-/1H+ exchange, we observed positive (Cl- efflux) or negative (Cl- influx) transient currents.

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Background and Assay Description: 
We have analyzed transporter symmetry by comparing chloride influx with chloride efflux. In both cases, we prepared proteoliposomes in Cl-free solution. For the influx assay, we activated Clc by a single solution exchange providing 20 mM chloride. This leads to a negative current, reflecting the chloride induced net movement of one negative charge into the vesicles. For the efflux assay, we have equilibrated the sensor in solution containing 20 mM chloride, followed by Clc activation by removing chloride.

Author: Nanion Technologies, in collaboration with Merritt Maduke, Stanford University School of Medicine
Target: EriC, ClcA, ClC-ecl
Mode of transport: H+/2Cl- exchange
Organism: Escherichia coli
Sample: Proteoliposomes from purified protein, LPR 20
Platform:   Icon N1   SURFE²R N1

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