• ENaC

    Cumulative dose-dependent block of ENaC by Amiloride, measured on the SyncroPatch 96, a predecessor model of the SyncroPatch 384PE

ENaC | Epithelial Sodium Channel | Amiloride-sensitive Sodium Channel

Epithelial sodium channels, member of the ENaC/Degenerin superfamily

ENaC is assembled as a heterotrimer composed of three homologous subunits α or δ, β, and γ. These subunits are encoded by four genes: SCNN1A, SCNN1B, SCNN1G, and SCNN1D. As a typical member of the ENaC/Degenerin superfamily, each of the subunits consists of two transmembrane helices and an extracellular loop.

ENaC is located in the apical membrane of polarized epithelial cells in particular in the kidney (primarily in the distal tubule), the lung, and the colon facilitating Na⁺ reabsorption across the apical membranes of epithelial cells.


Data and Applications

ENaC - Epithelia Sodium Channel Analysis

SP96 Enac Amiloride

icon sp96   SyncroPatch 96 (a predecessor model of SyncroPatch 384PE) data and applications:

The data present cumulative dose-dependent block of ENaC by Amiloride. Currents were obtained performing ramps -100mV/100mV every 2 s from a Vhold=-40mV. The large window shows the effect of increasing concentrations (1 µM, 10µM, 100µM) of Compound on the ENaC currents. The IC50 with Amiloride was 2.3 µM. Data were collected with a success rate of ~70 %.




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