GABAA Receptor Alpha 1 Beta 2 Gamma 2
Family:
GABA Receptors (Cys-loop ligand-gated ion channels)
Subgroups:
The GABA receptor family has further been divided into GABAA, GABAB and GABAA-ρ (also called GABAC) receptors.
Topology:
As typical for Cys-loop receptors, generally four transmembrane spanning domains TM1-TM4 form one receptor subunit, five or four subunits form a pore, several heteromeric combinations are known.
GABAA: Oligomerization of typically five subunits, heteromeric combinations are known of α subunits (GABRA1 - GABRA6), β subunits (GABRB1 - GABRB3) and γ subunits (GABRG1 - GABRG3), as well as δ (GABRD), an ε (GABRE), a π (GABRP), and a θ (GABRQ). The most common type is (α1)2(β2)2(γ2), minimal requirement is the inclusion of α and β subunits.
GABAB: Heterodimer (GABBR1, GABBR2)
GABAA-ρ: Oligomerization of five subunits, heteromeric combinations of three GABAA-ρ subunits are known (GABRR1, GABRR2, GABRR3)
Regulation and Function:
GABAA and GABAA-ρ receptors are ligand-gated ion channels (ionotropic receptors); whereas GABAB receptors are G protein-coupled receptors (metabotropic receptors). All respond to the neurotransmitter gamma-aminobutyric acid (GABA), the main inhibitory transmitter in the mammalian CNS.
Data and Applications
GABAA Receptor (α1β2γ2) - Success Rates
SyncroPatch 384PE (a predecessor model of SyncroPatch 384) data and applications:
Cells were kindly provided by Bsys.
Statistic of hGABAA α1β2γ2 cells recorded on one NPC-384 1-hole (1x) patch clamp chip. 57 % of the cells on one NPC-384 chip had seal resistance > 1 GOhm at the beginning and 48% at the end of the experiment. Access (RSeries) was good with 80% of cells with RSeries <20 MOhm at the start of the experiment.
GABAA Receptor (a1b2g2) - Reliable Compound Application
Port-a-Patch data and applications:
Ligand dependent activation of GABAA receptors can be recorded with the Laminar Flow Chamber with approx. 100 ms time constant for 10 μM GABA. Concentration dependent activation and desensitization of GABAA (α1β2γ2) receptors was obtained by applying 1, 3 and 10 μM GABA for 2.5 s at a time interval of 20 s.
GABAA Receptor (a1b2g2) - Activation
Patchliner data and applications:
Shown is concentration dependent activation of GABAA (α1β2γ2) receptors. Shown is the average concentration response curve for an average of 7 cells and the example traces from one cell.
Application Notes
GABAA (a1b2g2) - "Rapid agonist applications in precise intervals (GABAA) "
Patchliner application note: 
(1.5 MB)
GABAA (a1b2g2) - "Patchliner - Investigating the pharmacology of GABAA receptors expressed in HEK293 cells"
Patchliner application note: (0.7 MB)
Publications
2016 - Automated Patch Clamp Meets High-Throughput Screening: 384 Cells Recorded in Parallel on a Planar Patch Clamp Module
SyncroPatch 384PE (a predecessor model of SyncroPatch 384i) publication in Journal of Lab Automation (2016)
Authors:
Obergrussberger A., Brüggemann A., Goetze T.A., Rapedius M., Haarmann C., Rinke I., Becker N., Oka T., Ohtsuki A., Stengel T., Vogel M., Steindl J., Mueller M., Stiehler J., George M., Fertig N.
2011 - State-of-the-art automated patch clamp devices: heat activation, action potentials, and high throughput in ion channel screening
Port-a-Patch, 
Patchliner and SyncroPatch 96 (a predecessor model of SyncroPatch 384PE) publication in Frontiers in Pharmacology (2011)
Authors:
Stoelzle S., Obergrussberger A., Brüggemann A., Haarmann C., George M., Kettenhofen R., Fertig N.
GABAA: Further Readings
2021 - Cell engineering method using fluorogenic oligonucleotide signaling probes and flow cytometry
Patchliner publication in Biotechnology Letters (2021)
Authors:
Shekdar K., Langer J., Venkatachalan S., Schmid L., Anobile J., Shah P., Lancaster A., Babich O., Dedova O., Sawchuck D.
2020 - Novel Expression of GABAA Receptors on Resistance Arteries That Modulate Myogenic Tone
SyncroPatch 96 (a predecessor model of the SyncroPatch 384i) Publication in Journal of Vascular Research (2020)
Authors:
Yim P.D., Gallos G., Lee-Kong S.A., Dan W., Wu A.D., Xu D., Berkowitz D.E., Emala C.W.
2016 - Automated Patch Clamp Meets High-Throughput Screening: 384 Cells Recorded in Parallel on a Planar Patch Clamp Module
SyncroPatch 384PE (a predecessor model of SyncroPatch 384i) publication in Journal of Lab Automation (2016)
Authors:
Obergrussberger A., Brüggemann A., Goetze T.A., Rapedius M., Haarmann C., Rinke I., Becker N., Oka T., Ohtsuki A., Stengel T., Vogel M., Steindl J., Mueller M., Stiehler J., George M., Fertig N.
2015 - Novel screening techniques for ion channel targeting drugs
Patchliner, SyncroPatch 384PE (a predecessor model of SyncroPatch 384i) and 
CardioExcyte 96 publication in Channels (2015)
Authors:
Obergrussberger A., Stölzle-Feix S., Becker N., Brüggemann A., Fertig N., Möller C.
2014 - Crystal structure of a human GABAA receptor
Port-a-Patch publication in Nature (2014)
Authors:
Miller P.S., Aricescu A.R.
2012 - Characterizing Human Ion Channels in Induced Pluripotent Stem Cell-Derived Neurons
Patchliner publication in Journal of Biomolecular Screening (2012)
Authors:
Haythornthwaite A, Stoelzle S, Hasler A, Kiss A, Mosbacher J, George M, Brüggemann A, Fertig N.
2009 - Port-a-Patch and Patchliner: High fidelity electrophysiology for secondary screening and safety pharmacology
Port-a-Patch and Patchliner publication in Combinatorial Chemistry & High Throughput Screening (2009)
Authors:
Farre C., Haythornthwaite A., Haarmann C., Stoelzle S., Kreir M., George M., Brüggemann A., Fertig N.
2007 - Automated ion channel screening: patch clamping made easy
Port-a-Patch and Patchliner publication in Expert Opinion Therapeutic Targets (2007)
Authors:
Farre C., Stoelzle S., Haarman C., George M., Brueggemann A., Fertig N.
2006 - Microchip technology for automated and parallel patch clamp recording
Port-a-Patch and Patchliner publication in Small Journal (2006)
Authors:
Brüggemann A., Stoelzle S., George M., Behrends J.C., Fertig N.