• KCa1.1

    Single channel currents of BK channels as recorded from CHO cells in the cell attached mode on the Port-a-Patch

KCa1.1 | BK | Maxi-K | Calcium-activated Potassium Channel Subunit Alpha-1

Calcium-activated Potassium channels

Today, eight  human calcium-activated channels are known: KCa1.1 (also known as BK or Maxi-K), KCa2.1 (also known as SK1), KCa2.2 (also known as SK2), KCa2.3 (also known as SK3) , KCa3.1 (also known as IK or SK4), KCa4.1, KCa4.2, KCa5.1

KCa channels are made up of two different subunits, alpha and beta. The alpha subunit contains six or seven trans-membrane regions and forms homo- or heter-tetramers. The beta subunit has a regulative function and contains 2 trans-membrane regions.

This family of ion channels is, for the most part, activated by intracellular Ca2+. However, some of these channels (the KCa4 and KCa5 channels) are responsive instead to other intracellular ligands, such as Na+, Cl, and pH. Furthermore, multiple members of family are both ligand and voltage activated.

Data and Applications

Neurons (Primary Hippocampal Granule Cell) - Current Traces

icon pap   Port-a-Patch data and applications:

Recordings from acutely isolated hippocampal granule cells show BK- and CaV currents. Whole cell currents were obtained by depolarizing voltage pulses from a holding potential of −80 mV.

KCa1.1 (BK) - Single Channel Recordings

application kca11 1

icon pap   Port-a-Patch data and applications:

Single channel recordings of BK channels as recorded from CHO cells in the cell attached mode at +60 mV (top), +40 mV (middle) and 0 mV (lowest trace).


KCa1.1 (BK) - Intracellular Second Messengers

icon pap   Port-a-Patch data and applications:p24 2 InternalApp

Currents mediated by BK channels expressed in CHO cells were studied using the Internal Perfusion System. Currents were elicited by a voltage step from -80 mV to +80 mV before and after adding an internal solution containing a higher concentration of free Ca2+.

KCa1.1 (BK) - Activation by Internal Calcium

BK verticalicon pl   Patchliner data and applications: 

Top: BK (KCa1.1) current voltage relationships in a single cell showing effects of changing the intracellular free Ca2+ concentration (15 nM, n = 9; 108 nM, n = 11; 316 nM, n = 11).

Bottom: Comparison of BK (KCa1.1) current voltage relationships obtained on a conventional patch clamp setup (closed circles, n = 10) and on the Patchliner (open circles, n = 11).
Data are taken from Milligan C.J. et al., Nature Protocols, 2009, 4(2), 244-255.




2015 - Organellar Transporters and Ion Channels - How to access their electrophysiology by using the SURFE2R technology and Planar Patch Clamp

Icon N1   SURFE²R N1 and   icon sp96   SyncroPatch 96 (a predecessor model of the SyncroPatch 384PE) and   icon pap   Port-a-Patch poster, GRC - Organellar Channels and Transporters 2015   logo pdf   (1.6 MB)


2019 - KCa1.1 and Kv1.3 channels regulate the interactions between fibroblast-like synoviocytes and T lymphocytes during rheumatoid arthritis

icon pap   Port-a-Patch publication in Arthritis Research & Therapy (2019)

Tanner M.R., Pennington M.W., Chauhan S.S., Laragione T., Gulko P.S., Beeton C.

2019 - Inhibition of Polyamine Biosynthesis Reverses Ca2+ Channel Remodeling in Colon Cancer Cells

icon pap   Port-a-Patch publication in Cancers (2019)

Gutiérrez L.G., Hernández-Morales M., Núñez L., Villalobos C.

2018 - Targeting KCa1.1 channels with a scorpion venom peptide for the therapy of rat models of rheumatoid arthritis

icon pap   Port-a-Patch publication in The Journal of Pharmacology and Experimental Therapeutics (2018)

Tanner M.R., Pennington R.W., Chamberlain B.H., Huq R., Gehrmann E.J., Laragione, T., Gulko P.S., Beeton C.

2014 - Multi-Generational Pharmacophore Modeling for Ligands to the Cholane Steroid-Recognition Site in the β1 Modulatory Subunit of the BK(Ca) Channel

icon pl  Patchliner publication in Journal of Molecular Graphics and Modelling (2014)

McMillan J.E., Bukiya A.N., Terrell C.L., Patil S.A., Miller D.D., Dopico A.M., Parrilla A.L.

2014 - KCNMA1 Encoded Cardiac BK Channels Afford Protection against Ischemia-Reperfusion Injury

icon pap  Port-a-Patch publication in PLoS One (2014)

Soltysinska E., Bentzen B.H., Barthmes M., Hattel H., Thrush A.B., Harper M.E., Qvortrup K., Larsen F.J., Schiffer T.A., Losa-Reyna J., Straubinger J., Kniess A., Thomsen M.B., Brüggemann A., Fenske S., Biel M., Ruth P., Wahl-Schott C., Boushel R.C., Olesen S.P., Lukowski R.

2009 - Robotic multiwell planar patch-clamp for native and primary mammalian cells

icon pl  Patchliner publication in Nature Protocols (2009)

Milligan C.J., Li J., Sukumar P., Majeed Y., Dallas M.L., English A., Emery P., Porter K.E., Smith A.M., McFadzean I., Beccano-Kelly D., Bahnasi Y., Cheong A., Naylor J., Zeng F., Liu X., Gamper N., Jiang L., Pearson H.A., Peers C., Robertson B., Beech D.J.

2006 - Microchip technology for automated and parallel patch clamp recording

icon pap  Port-a-Patch and   icon pl   Patchliner publication in Small Journal (2006)

Brüggemann A., Stoelzle S., George M., Behrends J.C., Fertig N.



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