MscL - Pressure Clamp Experiments
Port-a-Patch and
Vesicle Prep Pro data and applications:
The Port-a-Patch and the Suction Control Pro was used to study the effect of pressure on functional MscL protein reconstituted in GUVs. Using symmetrical KCl solutions, MscL gating was observed at -30 mBar and -60 mBar (holding potential + 30 mV), shown as outward currents in the data traces.