• Na+/K+-ATPase

    The conductance of the Na+/K+ATPase is measured on the SURFE²R N1 in the presence of ATP, Na+ and K+, or in the presence of ATP and Na+.

Na+/K+-ATPase - Sodium/Potassium-Exchanging ATPase

Family:
P-type ATPase (P-ATPase) Superfamily

Subgroups and Members:
P-type ATPases can be divided into five subfamilies (types)
Type I consists of the transition/heavy metal ATPases.
Type II ATPases (specific for Na+,K+, H+ Ca2+, Mg2+ and phospholipids) predominate in eukaryotes. SERCA and  Na+/K+ ATPases are member of this group.
There are 3 major paralogs, SERCA1-3, which are expressed at various levels in different cell types: SERCA1 - SERCA3, encoded by the genes  ATP2A1 - ATP2A3.
Type III ATPases contains the plasma membrane H+-ATPases from plants and fungi.
Type IV ATPases have been shown to be involved in the transport of phospholipids
Type V ATPases have unknown specificity.

Topology:
Many of these protein complexes are multisubunit with a large subunit serving the primary ATPase and ion translocation functions. Many eukaryotic P-type ATPases are monomeric or homodimeric enzymes of the catalytic subunit that hydrolyzes ATP. They contain the aspartyl phosphorylation site and catalyzes ion transport. The Na+/K+-ATPases, the Ca2+-ATPases and the (fungal) H+-ATPases of higher organisms exhibit 10 transmembrane α helical spanners (TMSs).

Function:
P-type ATPases are α-helical bundle primary transporters named based upon their ability to catalyze auto- (or self-) phosphorylation of a key conserved aspartate residue within the pump and their energy source, adenosine triphosphate (ATP). Most members of this transporter superfamily catalyze cation uptake and/or efflux, however one subfamily is involved in flipping phospholipids to maintain the asymmetric nature of the biomembrane.

Data and Applications

Sodium-Potassium ATPase - Analysis

Surfer NaK ATPaseIcon N1   SURFE2R N1 data and applications:

The Sodium-Potassium ATPase, also known as Na+/K+ pump is responsible for the active transport of Na+ and K+ in the cells containing relatively high concentrations of K+ ions but low concentrations of Na+ ions. The Na+/K+-ATPase helps maintain resting potential, avail transport, and regulate cellular volume. Here, we demonstrate the conductance of the pump, in the presence of ATP, Na+ and K+, or in the presence of ATP and Na+.

Posters

a.      P-bond hydrolysis driven transporters

Cookies make it easier for us to provide you with our services. With the usage of our services you permit us to use cookies.
More information Ok