• Na+/K+-ATPase

    The conductance of the Na+/K+ATPase is measured on the SURFE²R N1 in the presence of ATP, Na+ and K+, or in the presence of ATP and Na+.

Na+/K+-ATPase - Sodium/Potassium-Exchanging ATPase

Family:
P-type ATPase (P-ATPase) Superfamily

Subgroups and Members:
P-type ATPases can be divided into five subfamilies (types)
Type I consists of the transition/heavy metal ATPases.
Type II ATPases (specific for Na+,K+, H+ Ca2+, Mg2+ and phospholipids) predominate in eukaryotes. SERCA and  Na+/K+ ATPases are member of this group.
There are 3 major paralogs, SERCA1-3, which are expressed at various levels in different cell types: SERCA1 - SERCA3, encoded by the genes  ATP2A1 - ATP2A3.
Type III ATPases contains the plasma membrane H+-ATPases from plants and fungi.
Type IV ATPases have been shown to be involved in the transport of phospholipids
Type V ATPases have unknown specificity.

Topology:
Many of these protein complexes are multisubunit with a large subunit serving the primary ATPase and ion translocation functions. Many eukaryotic P-type ATPases are monomeric or homodimeric enzymes of the catalytic subunit that hydrolyzes ATP. They contain the aspartyl phosphorylation site and catalyzes ion transport. The Na+/K+-ATPases, the Ca2+-ATPases and the (fungal) H+-ATPases of higher organisms exhibit 10 transmembrane α helical spanners (TMSs).

Function:
P-type ATPases are α-helical bundle primary transporters named based upon their ability to catalyze auto- (or self-) phosphorylation of a key conserved aspartate residue within the pump and their energy source, adenosine triphosphate (ATP). Most members of this transporter superfamily catalyze cation uptake and/or efflux, however one subfamily is involved in flipping phospholipids to maintain the asymmetric nature of the biomembrane.

Data and Applications

Sodium-Potassium ATPase - Analysis

Surfer NaK ATPaseIcon N1   SURFE2R N1 data and applications:

The Sodium-Potassium ATPase, also known as Na+/K+ pump is responsible for the active transport of Na+ and K+ in the cells containing relatively high concentrations of K+ ions but low concentrations of Na+ ions. The Na+/K+-ATPase helps maintain resting potential, avail transport, and regulate cellular volume. Here, we demonstrate the conductance of the pump, in the presence of ATP, Na+ and K+, or in the presence of ATP and Na+.

Posters

Publications

2020 - Label-Free Bioelectrochemical Methods for Evaluation of Anticancer Drug Effects at a Molecular Level

Icon 96SE   SURFE²R 96SE and   Icon N1    SURFE²R N1 Review article in Frontiers in Sensors (2020)

Authors: 
Tadini-Buoninsegni F.,Palchetti I.

2017 - Identification of Na+/K+-ATPase inhibition-independent proarrhythmic ionic mechanisms of cardiac glycosides

icon pl   Patchliner publication in Nature Scientific Reports (2017)

Authors:
Koh C.H., Wu J., Chung Y.Y., Liu Z., Zhang R.R., Chong K., Korzh V., Ting S., Oh S., Shim W., Tian H.Y., Wei H.

2017 - Effect of cisplatin on the transport activity of PII-type ATPases

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Metallomics (2017)

Authors:
Tadini-Buoninsegni F., Sordi G, Smeazzetto S, Natile G, Arnesano F.

2017 - Cytotoxic effect of zinc oxide nanoparticles on murine photoreceptor cells via potassium channel block and Na+/K+‐ATPase inhibition

icon pap   Port-a-Patch publication in Cell Proliferation (2017)

Authors:
Chen C. Bu W., Ding H., Li Q., Wang D., Bi H., Guo D.

2010 - Electrophysiological characterization of ATPases in native synaptic vesicles and synaptic plasma membranes

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Biochemical Journal (2010)

Authors:
Obrdlik P., Diekert K., Watzke N., Keipert C., Pehl U., Brosch C., Boehm N., Bick I., Ruitenberg M., Volknandt W., Kelety B.

2009 - Inhibitory effect of Pb2+ on the transport cycle of the Na+,K+-ATPase

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Chemical Research in Toxicology (2009)

Authors:
Gramigni E., Tadini-Buoninsegni F., Bartolommei G., Santini G., Chelazzi G., Moncelli M.R.

2009 - Electrogenic ion pumps investigated on a solid supported membrane: comparison of current and voltage measurements

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Langmuir (2009)

Authors:
Bartolommei G., Moncelli M.R., Rispoli G., Kelety B., Tadini-Buoninsegni F.

2008 - Effect of Clotrimazole on the Pump Cycle of the Na,K-ATPase

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Biophysical Journal (2008)

Authors:
Bartolommei G., Devaux N., Tadini-Buoninsegni F., Moncelli M., Apell H.-J.

1999 - Charge Translocation by the Na+/K+-ATPase Investigated on Solid Supported Membranes: Cytoplasmic Cation Binding and Release

Icon N1   SURFE²R-technology (custom-built system) publication in Biophysical Journal (1999)

Authors:
Pintschovius J., Fendler K., Bamberg E.

1999 - Charge Translocation by the Na/K-ATPase Investigated on Solid Supported Membranes: Rapid Solution Exchange with a New Technique

Icon N1   SURFE²R-technology (custom-built system) publication in Biophysical Journal (1999)

Authors:
Pintschovius J., Fendler K.

1993 - Charge transport by ion translocating membrane proteins on solid supported membranes

Icon N1   SURFE²R-technology (custom-built system) publication in Biophysical Journal (1993)

Authors:
Seifert K., Fendler K., Bamberg E.

a.      P-bond hydrolysis driven transporters

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