2021 - Reliable identification of cardiac liability in drug discovery using automated patch clamp: Considerations and best practices for high throughput recordings of NaV 1.5

   Patchliner and     SyncroPatch 384i (a predecessor model of SyncroPatch 384) Physiology 2021     (2 MB)

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Abstract:

For reliable identification of cardiac safety risk, compounds should be screened for effects on cardiac ion channels in addition to hERG including Na_V1.5 and Ca_V1.2 Automated patch clamp (instruments are increasingly adopted for cardiac safety measurements but cross site and cross platform comparisons of IC₅₀ values has identified the need for standardized
protocols for reliable pharmacology In this study, we identified different parameters that might affect IC₅₀s of compounds on Na_V1.5 peak and late currents recorded using APC.


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