NaV1.7 - "Patch clamp recordings of hNaV1.7 on Nanion’s Port-a-Patch"
Port-a-Patch application note (0.7 MB)
Cells were kindly provided by Anaxon
The NaV1.7 gene (SCN9A) encodes a voltage-gated sodium (NaV) channel, primarily expressed in the peripheral nervous system and has been isolated from rat dorsal root ganglion (DRG) neurons, human medullary thyroid cancer cells (hNE-Na) and PC12 cells. Different NaV channels play a key role in modulation of action potentials in the central and peripheral nervous systems. In particular, the fast upstroke of the action potential is mediated by NaV channels. NaV channels are in part characterized by their TTX-sensitivity (TTX-resistant [TTXr], TTX-sensitive [TTXs]). NaV1.7 is a TTXs channel and is sensitive to TTX in the nanomolar range. The role of hNaV1.7 has yet to be fully elucidated but is proposed to play an important role in nociception and pain sensing. NaV1.7 has been implicated to play a role in disease pain states, in particular inflammatory pain and hypersensitivity to heat following burn injury. Common to many of the voltagegated ion channels, a number of compounds display a higher affinity for the inactivated state of the channel. For this reason, it is important to be able to reliably record both activation and inactivation kinetics of the channel. In this Application Note we present data using the Port-a-Patch characterizing CHO cells stably expressing hNaV1.7. The hNaV1.7 activation and inactivation properties and TTX sensitivity are consistent with those reported in the literature.