NaV1.9 | sodium voltage-gated channel alpha subunit 11

Family:
Sodium channels

Subgroups:
NaV1.1-1.9

Topology:
Alpha subunits consist of four homologous domains (I-IV) with six transmembrane alpha helices (S1–S6) and a pore-forming loop. One a subunit may associate with 1 or 2 b subunits to make up the channel.

NaV1.9: Background Information

NaV1.9 is largely expressed in small-diameter nociceptors of the dorsal root ganglion and trigeminal ganglion neurons, but is also found in intrinsic myenteric neurons. Like Nav1.7 and Nav1.8, Nav1.9 plays a role in pain perception, having been linked to the perception of inflammatory, neuropathic, and cold-related pain. It does this primarily through its ability to lower the threshold potential of the neuron, allowing for an increase in action potential firing that leads to hyperexcitability of the neuron and increased pain perception.This channel NaV1.9 is a TTX-resistant sodium channel.

Gene:
SCN11A

Human Protein:
UniProt Q9UI33

Tissue:
Brain, olfactory bulb, hippocampus, cerebellar cortex, spinal cord, small intestine, spleen, testis, bladder, and placenta

Function/ Application:
Excitability of neurons (lowering the threshold potential), nociceptive transmission

Pathology:
Episodic pain syndrome, familial, 3 (FEPS3), Pathogenic Neuropathy hereditary sensory and autonomic 7 (HSAN7), Diarrhea, Dysautonomia, Hyperhidrosis

Accessory subunits:SCN1B, SCN2B and SCN3B

Interaction:
Calmodulin 1, Calmodulin 2, Calmodulin 3, spectrin, Neurofascin, CACNA1H, CACNA1I, Kinesin Family Member 5C, Ryanodine Receptor 1, Ryanodine Receptor 2

Modulator:
Tetracaine, Nifedipine, Carbamazepine, Vinpocetine, Amiloride hydrochloride

Assays:
Patch Clamp: whole cell, perforated patch

Particularities:
NaV channel analysis requires GigaOhm seals and a stable and low access resistance

Recommended Reviews:
International Union of Pharmacology. XLVII. Nomenclature and Structure-Function Relationships of Voltage-Gated Sodium Channels. Pharmacol Rev 57: 397–409, Catterall, et al. 2005

Data and Applications

NaV1.9 - Pharmacology

NaV1.9 Dataset PE 1icon sp96   SyncroPatch 384/768 PE data and applications:
Cells were kindly provided by Icagen.

Screenshots of the PatchControl 384 software showing hNaV1.9 current traces in response to a voltage step protocol. Measured on the SyncroPatch 384PE using perforated patch methodology (Escin) and multi-hole chips (4 holes per well), the success rate of valuable data for the analysis was  86%. NaV1.9 is TTX insensitive. The IC50 value of lidocaine of the peak current was determined as 350 µM (Literature: 356 µM), the IC50 value of tetracaine of the peak current was determined as 12.5 µM (Literature: 32 µM).

Webinars and Movies

2018 - Biophysical and Pharmacological Characterization of Voltage-Gated Sodium Channels Involved in Pain Pathways

icon sp96   SyncroPatch 384PE Oral Presentation Video

Presenter: 
Dr. Markus Rapedius, Senior Scientist, Nanion Technologies

2018 - Biophysical and Pharmacological Characterization of Voltage-Gated Sodium Channels Involved in Pain Pathways

icon sp96   SyncroPatch 384PE Oral Presentation Video

Presenter: 
Dr. Markus Rapedius, Senior Scientist, Nanion Technologies

2018 - Biophysical and Pharmacological Characterization of Voltage-Gated Sodium Channels Involved in Pain Pathways

icon sp96   SyncroPatch 384PE Oral Presentation Video

Presenter: 
Dr. Markus Rapedius, Senior Scientist, Nanion Technologies

Posters

2018 - Investigating pain pathways by inhibition of voltage-gated sodium channels

icon sp96   SyncroPatch 384PE and   icon pl   Patchliner poster, FENS Meeting 2018  logo pdf   (2.5 MB)

 

 

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