NCX1 - Sodium-Calcium Exchanger Member 1 - SLC8A1

Calcium-Cation Antiporter family (CaCA)

The CaCA superfamily is composed of five families: K+-independent Na+/Ca2+ exchangers (NCXs), cation/Ca2+ exchangers (CCXs), YbrG transporters and cation exchangers (CAXs). Phylogenetic and alignment studies indicate that one of the mammalian NCKXs, NCKX6, and its related proteins form a unique group, designated cation/Ca2+ exchangers (CCXs)

Proteins of the CaCA family are found ubiquitously, having been identified in animals, plants, yeast, archaea and divergent bacteria. They exhibit widely divergent sequences, and several have been shown to have arisen by a tandem intragenic duplication event. The most conserved portions of this repeat element, α1 and α2, are found in putative transmembrane regions 2-3 and transmembrane regions 7-8. Members of this family contain nine transmembrane regions in mammals.

All of the characterized animal proteins catalyze Ca2+:Na+ exchange although some also transport K+. The NCX plasma membrane proteins exchange 3 Na+ for 1 Ca2+. Mammalian Na+/Ca2+ exchangers exist as three isoforms NCX1-3 which are about 70% identical to each other.

Data and Applications

NCX1 - High Throughput Screening on the SURFE2R 96SE


Icon N1   SURFE2R 96SE data and applications:
Cells kindly provided by Charles River.

NCX1 over-expressed in HEK cells was recorded on the SURFE2R 96SE. A membrane preparation of HEK cells expressing NCX1 was used on the SURFE2R 96SE. An activating buffer containing 100 μM Ca2+ was used to activate NCX1 (black and dark blue traces) after a negative control (0 Ca2+; light blue trace). NCX1 was activated three times after which a pharmacological experiment can be started to test for inhibitors. Wells are color-coded so that the user can get an overview of the succes of the experiment at a glance. Green wells depict resistance >500 MΩ, blue depicts wells have a resistance between 100 and 500 MΩ.

NCX1 - Effect of block of cardiac NCX on beat rate

Icon CE   NCX1 iCell SEA0400 CE96CardioExcyte data and applications:
Cells were kindly provided by Cellular Dynamics.

Block of NCX1 expressed in iCell® Cardiomyocytes2 by SEA 0400 caused a dose dependent increase in beat rate. 


NCX1 - Current traces recorded from Cor.4U Cardiomyocytes, iCell Cardiomyocytes and HEK cells

NCX1 Cor4U icell HEK

Icon N1   SURFE2R N1 data and applications:

Cardiac NCX1 was activated using 30 μM Ca2+ on the SURFE2R N1. NCX1 was recorded from Cor.4U® Cardiomyocytes, iCell® Cardiomyocytes2 or overexpressed in HEK cells. The calcium affinity was determined for NCX1 recorded from iCell® Cardiomyocytes2 and HEK cells, shown on the right.  


NCX1 - Block by SEA0400

NCX1 iCell HEK SEA0400

Icon N1   SURFE2R N1 data and applications:

Cardiac NCX1 expressed in iCell® Cardiomyocytesor HEK cells was blocked by increasing concentrations of SEA0400, a specific blocker of NCX.  


NCX1 - Block by KB-R7943

NCX1 KBR7943 iCell Cor4U HEK

Icon N1   SURFE2R N1 data and applications:

Cardiac NCX1 expressed in iCell® Cardiomyocytes2, Cor.4U® Cardiomyocytes or HEK cells was blocked by increasing concentrations of KB-R7943.   




2021 - Drug Effects on human Na+/Ca2+ Exchanger and Implications for Drug Development

Icon CE   FLEXcyte 96 and   Icon N1  SURFE²R N1 and   Icon 96SE   SURFE²R 96SE poster, Biophysical Society Meeting 2021  logo pdf   (1.3 MB)

2020 - A novel approach to detect electrogenic transporter activity in intact cells applied to investigate iPSC derived cardiomyocytes and neurons

 Icon N1   SURFE²R N1 and   Icon 96SE   SURFE²R 96SE poster, 64th Annual Meeting of the Biophysical Society   logo pdf   (1.6 MB)

2018 - Transported by light: optogenetic control of NCX1

Icon N1   SURFE²R N1 poster, Biophysics Annual Meeting 2018  logo pdf   (2.2 MB)

2018 - Label-free analysis of Na+/Ca2+- exchanger (NCX) isolated from iPSC-derived cardiomyocytes

Icon N1   SURFE²R N1 and   Icon 96SE   SURFE2R 96SE poster, Europhysiology Meeting 2018  logo pdf   (1.5 MB)

2017 - High throughput real-time measurement of electrogenic membrane transport driven by the SLC transporters PepT1 and OcT2

Icon 96SE   SURFE²R SE96 poster, Biophysics congress 2017  logo pdf   (1.8 MB)


2016 - Electrophysiological characterization of the archaeal transporter NCX_Mj using solid supported membrane technology

Icon N1  SURFE²R N1 and   icon vpp   Vesicle Prep Pro publication in Journal of General Physiology (2016)

Barthmes M., Liao J., Jiang Y., Brüggemann A., Wahl-Schott C.

2006 - Transporter assays using solid supported membranes: a novel screening platform for drug discovery

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in ASSAY and Drug Development Technologies (2006)

Kelety B., Diekert K., Tobien J., Watzke N., Dörner W., Obrdlik P., Fendler K.

2006 - Establishment of Cell-Free Electrophysiology for Ion Transporters: Application for Pharmacological Profiling

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Journal of Biomolecular Screening (2006)

Geibel S., Flores-Herr N., Licher T., Vollert H.

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