NCX1 - Sodium-Calcium Exchanger Member 1 - SLC8A1

Family:
Calcium-Cation Antiporter family (CaCA)

Subgrops:
The CaCA superfamily is composed of five families: K+-independent Na+/Ca2+ exchangers (NCXs), cation/Ca2+ exchangers (CCXs), YbrG transporters and cation exchangers (CAXs). Phylogenetic and alignment studies indicate that one of the mammalian NCKXs, NCKX6, and its related proteins form a unique group, designated cation/Ca2+ exchangers (CCXs)

Topology:
Proteins of the CaCA family are found ubiquitously, having been identified in animals, plants, yeast, archaea and divergent bacteria. They exhibit widely divergent sequences, and several have been shown to have arisen by a tandem intragenic duplication event. The most conserved portions of this repeat element, α1 and α2, are found in putative transmembrane regions 2-3 and transmembrane regions 7-8. Members of this family contain nine transmembrane regions in mammals.

Function:
All of the characterized animal proteins catalyze Ca2+:Na+ exchange although some also transport K+. The NCX plasma membrane proteins exchange 3 Na+ for 1 Ca2+. Mammalian Na2+/Ca2+ exchangers exist as three isoforms NCX1-3 which are about 70% identical to each other.

Data and Applications

NCX1 - High Throughput Screening on the SURFE2R 96SE

NCX1 HEK SURFE2R 96SE

Icon N1   SURFE2R 96SE data and applications:
Cells kindly provided by Charles River.

NCX1 over-expressed in HEK cells was recorded on the SURFE2R 96SE. A membrane preparation of HEK cells expressing NCX1 was used on the SURFE2R 96SE. An activating buffer containing 100 μM Ca2+ was used to activate NCX1 (black and dark blue traces) after a negative control (0 Ca2+; light blue trace). NCX1 was activated three times after which a pharmacological experiment can be started to test for inhibitors. Wells are color-coded so that the user can get an overview of the succes of the experiment at a glance. Green wells depict seal resistance >500 MΩ, blue depicts wells have a seal resistance between 100 and 500 MΩ.

NCX1 - Effect of block of cardiac NCX on beat rate

Icon CE   NCX1 iCell SEA0400 CE96CardioExcyte data and applications:
Cells were kindly provided by Cellular Dynamics.

Block of NCX1 expressed in iCell® Cardiomyocytes2 by SEA 0400 caused a dose dependent increase in beat rate. 

 

NCX1 - Current traces recorded from Cor.4U Cardiomyocytes, iCell Cardiomyocytes and HEK cells

NCX1 Cor4U icell HEK

Icon N1   SURFE2R N1 data and applications:

Cardiac NCX1 was activated using 30 μM Ca2+ on the SURFE2R N1. NCX1 was recorded from Cor.4U® Cardiomyocytes, iCell® Cardiomyocytes2 or overexpressed in HEK cells. The calcium affinity was determined for NCX1 recorded from iCell® Cardiomyocytes2 and HEK cells, shown on the right.  

 

NCX1 - Block by SEA0400

NCX1 iCell HEK SEA0400

Icon N1   SURFE2R N1 data and applications:

Cardiac NCX1 expressed in iCell® Cardiomyocytesor HEK cells was blocked by increasing concentrations of SEA0400, a specific blocker of NCX.  

 

NCX1 - Block by KB-R7943

NCX1 KBR7943 iCell Cor4U HEK

Icon N1   SURFE2R N1 data and applications:

Cardiac NCX1 expressed in iCell® Cardiomyocytes2, Cor.4U® Cardiomyocytes or HEK cells was blocked by increasing concentrations of KB-R7943.  

 

Posters

Publications

2016 - Electrophysiological characterization of the archaeal transporter NCX_Mj using solid supported membrane technology

Icon N1  SURFE²R N1 and   icon vpp   Vesicle Prep Pro publication in Journal of General Physiology (2016)

Authors: 
Barthmes M., Liao J., Jiang Y., Brüggemann A., Wahl-Schott C.

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