P2X2/ P2X3 - Application of ATP on the Patchliner
Patchliner data and applications:
Cells were kindly supplied by Evotec AG, Hamburg, Germany
P2X2/3 recorded from 1321N1 cells on the Patchliner. P2X2/3 was activated by increasing concentrations of ATP with an EC50 = 7.8 ± 1.0 µM (n = 10). ATP was applied for approximately 600 ms using the stacked solutions function of the Patchliner (holding potential = -80mV).