• P2X7

    BzATP concentration response curve obtained from wild-type P2X7 receptors as measured on the Patchliner.

P2X7 | P2X Purinoceptor 7

Purinergic Receptors P2X

The P2X family belongs to the P2 receptors superfamily which has further been divided into five subclasses: P2X, P2Y, P2Z, P2U, and P2T. The ligand-gated purinergic receptor class P2X contains seven members (P2X1 - P2X7).

One molecule is composed of only two transmembrane domains and contains a large extracellular loop and intracellular carboxyl and amino termini

Regulation and Function:
P2X receptors are ligand-gated ion channels and are activated by extracellular ATP. Greatly distributed in neurons and glial cells, P2X receptors mediate a large variety of responses including fast transmission at central synapses, contraction of smooth muscle cells, platelet aggregation, macrophage activation, and apoptosis.

Data and Applications

P2X7 - Application of BzATP

P2X7icon pl   Patchliner data and applications:
Data are taken from Milligan C.J. et al., Nature Protocols, 2009, 4(2), 244-255.

BzATP concentration response curve obtained from wild-type P2X7 receptors. Data are fite to the Hill equation with an EC50 of 69.7 ± 7.5 μM. The inset show representative currents evoked by BzATP (3 − 100 μM). Holding potential was −60 mV.


2020 - A P2RX7 single nucleotide polymorphism haplotype promotes exon 7 and 8 skipping and disrupts receptor function

icon pl   Patchliner publication in The Faseb Journal (2020)

Skarratt K.K., Gu B.J., Lovelace M.D., Milligan C.J., Stokes L., Glover R., Petrou S, Wiley J.S., Fuller S.J.

2019 - Automated Planar Patch-Clamp Recording of P2X Receptors

icon pl   Patchliner book chapter in Ion Channels (2020)

Milligan, C. J., Jiang L.H.

2017 - Purinergic receptors P2RX4 and P2RX7 in familial multiple sclerosis

icon pl  Patchliner publication in Human Mutation (2017)

Sadovnick A.D., Gu B.J., Traboulsee A.L., Bernales C.Q., Encarnacion M., Yee I.M., Criscuoli M.G., Huang X., Ou A., Milligan C.J., Petrou S, Wiley J.S, Vilariño-Güell C.

2012 - Alternative splicing of the N-terminal cytosolic and transmembrane domains of P2X7 controls gating of the ion channel by ADP-ribosylation

icon pap   Port-a-Patch publication in PLoS ONE (2012)

Schwarz N., Drouot L., Nicke A., Fliegert R., Boyer O., Guse A.H., Haag F., Adriouch S., Koch-Nolte F.

2009 - Robotic multiwell planar patch-clamp for native and primary mammalian cells

icon pl  Patchliner publication in Nature Protocols (2009)

Milligan C.J., Li J., Sukumar P., Majeed Y., Dallas M.L., English A., Emery P., Porter K.E., Smith A.M., McFadzean I., Beccano-Kelly D., Bahnasi Y., Cheong A., Naylor J., Zeng F., Liu X., Gamper N., Jiang L., Pearson H.A., Peers C., Robertson B., Beech D.J.

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