SERCA - Sarco/Endoplasmic Reticulum Calcium-ATPase

Family:
P-type ATPase (P-ATPase) Superfamily

Subgroups:
P-type ATPases can be divided into five subfamilies (types)
Type I consists of the transition/heavy metal ATPases.
Type II ATPases (specific for Na⁺,K⁺, H⁺ Ca²⁺, Mg²⁺ and phospholipids) predominate in eukaryotes. SERCA and Na⁺/K⁺ ATPases are member of this group.
There are 3 major paralogs, SERCA1-3, which are expressed at various levels in different cell types: SERCA1 - SERCA3, encoded by the genes ATP2A1 - ATP2A3.
Type III ATPases contains the plasma membrane H⁺-ATPases from plants and fungi.
Type IV ATPases have been shown to be involved in the transport of phospholipids
Type V ATPases have unknown specificity.

Topology:
Many of these protein complexes are multisubunit with a large subunit serving the primary ATPase and ion translocation functions. Many eukaryotic P-type ATPases are monomeric or homodimeric enzymes of the catalytic subunit that hydrolyzes ATP. They contain the aspartyl phosphorylation site and catalyzes ion transport. The Na+/K+-ATPases, the Ca²⁺-ATPases and the (fungal) H⁺-ATPases of higher organisms exhibit 10 transmembrane α helical spanners (TMSs).

Function:
P-type ATPases are α-helical bundle primary transporters named based upon their ability to catalyze auto- (or self-) phosphorylation of a key conserved aspartate residue within the pump and their energy source, adenosine triphosphate (ATP). Most members of this transporter superfamily catalyze cation uptake and/or efflux, however one subfamily is involved in flipping phospholipids to maintain the asymmetric nature of the biomembrane.

Publications

2021 - Stimulation of Ca2+‐ATPase Transport Activity by a Small‐Molecule Drug

Icon N1 SURFE²R ONE (a predecessor model of SURFE²R N1) publication in ChemMedChem(2021)

Authors:
Sordi G., Goti A., Young H.S., Palchetti I., Tadini‐Buoninsegni F.

2020 - Protein Adsorption on Solid Supported Membranes: Monitoring the Transport Activity of P-Type ATPases

Icon 96SE SURFE²R 96SE and Icon N1 SURFE²R N1 Review in Molecules (2020)

Authors:
Tadini-Buoninsegni F.

2020 - Label-Free Bioelectrochemical Methods for Evaluation of Anticancer Drug Effects at a Molecular Level

Icon 96SE SURFE²R 96SE and Icon N1 SURFE²R N1 Review article in Frontiers in Sensors (2020)

Authors:
Tadini-Buoninsegni F.,Palchetti I.

2020 - Homocysteine-induced electrical remodeling via the mediation of IP 3 R1/Nav1.5 signaling pathway

Port-a-Patch publication in American Journal of Translational Research (2020)

Authors:
Han L., Wu A., Li Q., Xia Z., Wu Y., Hong K., Xia Z., Li J.

2019 - Functional Reconstitution of Membrane Proteins Derived From Eukaryotic Cell-Free Systems

Icon N1 SURFE²R N1 and Icon Orbit Orbit 16 publication in Frontiers in Pharmacology (2019)

Authors:
Dondapati S.K., Lübberding H., Zemella A., Thoring L., Wüstenhagen D.A., Kubick S.

2018 - Drug Interactions With the Ca2+-ATPase From Sarco(Endo)Plasmic Reticulum (SERCA)

Icon 96SE SURFE²R 96SE Review article in Frontiers in Molecular Biosciences (2018)

Authors:
Tadini-Buoninsegni F., Smeazzetto S., Gualdani R., Moncelli M.R.

2017 - Mechanisms of charge transfer in human copper ATPases ATP7A and ATP7B

Icon N1 SURFE²R ONE (a predecessor model of SURFE²R N1) publication in IUBMB Life (2017)

Authors:
Tadini-Buoninsegni F., Smeazzetto S.

2017 - Effect of cisplatin on the transport activity of PII-type ATPases

Icon N1 SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Metallomics (2017)

Authors:
Tadini-Buoninsegni F., Sordi G, Smeazzetto S, Natile G, Arnesano F.

2017 - Conformational memory in the association of the transmembrane protein phospholamban with the sarcoplasmic reticulum calcium pump SERCA

Icon N1 SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Journal of Biological Chemistry (2017)

Authors:
Smeazzetto S., Armanious G.P., Moncelli M.R., Bak J.J., Lemieux M.J., Young H.S., Tadini-Buoninsegni F.

2015 - Hofmeister effect of anions on calcium translocation by sarcoplasmic reticulum Ca2+-ATPase

Icon N1 SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Nature Scientific Reports (2015)

Authors:
Tadini-Buoninsegni F., Moncelli M.R., Peruzzi N., Ninham B.W., Dei L., Lo Nostroa P.

2015 - A sulfur‐based transport pathway in Cu+‐ATPases

Icon N1 SURFE²R ONE (a predecessor model of SURFE²R N1) publication in EMBO Reports (2015)

Authors:
Mattle D., Zhang L., Sitsel O., Pedersen L.T., Moncelli M.R., Tadini-Buoninsegni F., Gourdon P., Rees D.C., Nissen P., Meloni G.

2014 - Anticancer Ruthenium(III) Complex KP1019 Interferes with ATP-Dependent Ca2+ Translocation by Sarco-Endoplasmic Reticulum Ca2+-ATPase (SERCA)

Icon N1 SURFE²R N1 publication in ChemMedChem (2014)

Authors:
Sadafi F.Z., Massai L., Bartolommei G., Moncelli M.R., Messori L., Tadini-Buoninsegni F.

2013 - Enhanced adsorption of Ca-ATPase containing vesicles on a negatively charged solid supported membrane for the investigation of membrane transporters

Icon N1 SURFE²R N1 publication in Langmuir (2013)

Authors:
Sacconi A., Moncelli M.R., Mergheri G., Tadini-Buoninsegni F.

2012 - Distinctive features of catalytic and transport mechanisms in mammalian sarco-endoplasmic reticulum Ca2+ ATPase (SERCA) and Cu+ (ATP7A/B) ATPases

Icon N1 SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Journal of Biological Chemistry (2012)

Authors:
Lewis D., Pilankatta R., Inesi G., Bartolommei G., Moncelli M.R., Tadini-Buoninsegni F.

Data and Applications

SERCA - IC50 for Cisplatin

Icon N1 SURFE²R N1 data and applications:

Ca²⁺-transport in SERCA is triggered by ATP hydrolysis. Using ATP concentration jumps, the authors determined the IC₅₀ of SERCA inhibition by cisplatin

a. P-bond hydrolysis driven transporters

SERCA