TRPM7 | Transient Receptor Potential Cation Channel Subfamily M Member 7
Family:
Transient receptor potential channels
Subgroups:
TRPC (TRPC1–TRPC7), TRPV (TRPV1-TRPV6), TRPA1, TRPM (TRPM1–TRPM8), TRPP (TRPP1–TRPP3, PKD1, PKDREJ, PKDL1–PKDL3), TRPML (TRPML1–TRPML3), TRPN
Topology:
Most TRP channels are composed of 6 transmembrane domains (helices) with intracellular N- and C-termini, non-selectively permeable to various cations
TRPM7: Background information
Gene:
SCN10A
Human Protein:
UniProt Q9Y5Y9
Tissue:
Brain (small diameter DRG neurons)
Function/ Application:
Excitability of neurons, nociceptive transmission
Pathology:
Neuropathic pain, Multiple Sclerosis
Accessory subunits:
b1, b2, b3
Interaction:
p11, PDZD2 and syntrophin-associated serine/threonine kinase (SASTK), Contactin, CAP-1A
Modulator:
Tetrodotoxin, lidocaine, benzocaine, A-887826, A-803467
Assays:
Patch Clamp: whole cell, room temperature, State- and use-dependence
Particularities:
Cell systems:
hNaV1.8 stably expressing CHO cell line (Charles River); rNaV1.8 stably expressed in ND7/23 cell line (EMD Millipore)
Recommended Reviews:International Union of Pharmacology. XLVII. Nomenclature and Structure-Function Relationships of Voltage-Gated Sodium Channels. Pharmacol Rev 57: 397–409, Catterall, et al. 2005
Data and Applications
TRPM7 - Current Traces
Port-a-Patch data and applications:
Shown are mTRPM7 (HEK293) raw current responses to a voltage ramp voltage procotol (−100 mV to 100 mV over 200 ms) recorded under control conditions and after subsequent internal perfusion with Mg2+ (left). Initally, currents showed some run-up before they became stable. The arrows in the timecourse on the right mark the timepoints for which the raw current traces are shown.
Application Notes
TRPM7 - "TRPM7 activation by internal sequestering of Mg2+ ions recorded on the Port-a-Patch"
Port-a-Patch application note: 
(0.8 MB)
Publications
2016 - Human T cells in silico: Modelling their electrophysiological behaviour in health and disease
Patchliner publication in Journal of Theoretical Biology (2016)
Authors:
Ehling P., Meuth P., Eichinger P., Hermann A.M., Bittner S., Pawlowski M., Pankratz S., Herty M., Budde T., Meuth S.G.