TRPM8 | Transient Receptor Potential Cation Channel M8
Family:
Transient receptor potential channels
Subgroups:
TRPC (TRPC1–TRPC7), TRPV (TRPV1-TRPV6), TRPA1, TRPM (TRPM1–TRPM8), TRPP (TRPP1–TRPP3, PKD1, PKDREJ, PKDL1–PKDL3), TRPML (TRPML1–TRPML3), TRPN
Topology:
Most TRP channels are composed of 6 transmembrane domains (helices) with intracellular N- and C-termini, non-selectively permeable to various cations
TRPM8: Background information
TRPM8, also known as the cold and menthol receptor 1 (CMR1) is the primary transducer of cold somatosensation in humans. The TRPM8 channel is a homotetramer, composed of four identical subunits with a transmembrane domain with six helices (S1–6). TRPM8 is an ion channel, upon activation it allows the entry of Na+ and Ca2+ ions to the cell that leads to depolarization and the generation of an action potential and it is activated by cold temperatures and cooling agents, such as menthol and icilin.
Gene:
TRPM8
Human Protein:
UniProt Q7Z2W7
Tissue:
Sensory neurons, dorsal root ganglia neurons, prostate, lung, bladder, prostatae tumors
Function/ Application:
Temperature transducer for ‘‘cold’’, proliferation, generation of an action potential
Pathology:
Cancer, thermoception, neuropathic pain, migrane, dentin sensitivity
Interaction:
TCAF1, PIP2, Gαq, Protein phosphatase 1, Ubiquitin specific peptidase 10
Modulator:
Cilin, eucalyptol, menthol, capsaicin, ethanol, linalool, geraniol, capsazepine , BCTC, cannabidiol
Assays:
Patch Clamp: whole cell, room temperature, State- and use-dependence
Particularities:
TRPM8 is a ligand-gated, intracellular pH- and thermo-sensitive ion channel
Recommended Reviews:International Union of Pharmacology. XLIII. Compendium of voltage-gated ion channels: transient receptor potential channels., Pharmacol Rev 55(4):591-6 Clapham, et al. 2003