TRPM8 | Transient Receptor Potential Cation Channel M8

Transient  receptor potential channels


Most TRP channels are composed of 6 transmembrane domains (helices) with intracellular N- and C-termini, non-selectively permeable to various cations

TRPM8: Background information

TRPM8, also known as the cold and menthol receptor 1 (CMR1) is the primary transducer of cold somatosensation in humans. The TRPM8 channel is a homotetramer, composed of four identical subunits with a transmembrane domain with six helices (S1–6). TRPM8 is an ion channel, upon activation it allows the entry of Na+ and Ca2+ ions to the cell that leads to depolarization and the generation of an action potential and it is activated by cold temperatures and cooling agents, such as menthol and icilin.


Human Protein:
UniProt Q7Z2W7

Sensory neurons, dorsal root ganglia neurons, prostate, lung, bladder, prostatae tumors

Function/ Application:
Temperature transducer for ‘‘cold’’, proliferation, generation of an action potential

Cancer, thermoception, neuropathic pain, migrane, dentin sensitivity

TCAF1, PIP2, Gαq, Protein phosphatase 1, Ubiquitin specific peptidase 10

Cilin, eucalyptol, menthol, capsaicin, ethanol, linalool, geraniol, capsazepine , BCTC, cannabidiol

Patch Clamp: whole cell, room temperature, State- and use-dependence

TRPM8 is a ligand-gated, intracellular pH- and thermo-sensitive ion channel

Recommended Reviews:
International Union of Pharmacology. XLIII. Compendium of voltage-gated ion channels: transient receptor potential channels., Pharmacol Rev 55(4):591-6 Clapham, et al. 2003  

Data and Applications

TRPM8 and Temperature Control

icon sp96   PE384 TRPM8SyncroPatch 384 data and applications:
Cells were kindly provided by Charles River Chantest

At RT, the TRPM8 current was activated using increasing concentrations of Menthol (left). Measurement site, cells and solutions can be accurately temperature controlled – in the presence of temperatures 38°C, 25°C, 18°C and 12 °C the current size of temperature regulated TRPM8 changes accordingly (right). TRPM8 is activated at temperatures < 25°C.


TRPM8 / TRPV1 - Pharmacology

icon pap   Port-a-Patch data and applications:p15 1 TRP 
TRPV1 data were kindly supplied by Dr. David Cohen, Oregon Health and Service University, Portland, OR, USA.

TRP channels recorded in HEK293 cells. Menthol (dark blue) activated the TRPM8 channels (shown on the left) and could be inhibited by genistein (blue). TRPV1 channels expressed in HEK293 cells (right) were stimulated by application of capsaicin.

TRPM8 - Activated by Menthol

AN Port a Patch TRPV1M8 2icon pap   Port-a-Patch data and applications:

External application of 300 µM menthol activated the TRPM8 channels expressed in HEK293 cells.




Application Notes


2019 - Purification of Functional Human TRP Channels Recombinantly Produced in Yeast

Icon Orbit Mini   Orbit mini publication in Cells (2019)

Zhang L., Wang K., Klaerke D.A., Calloe K., Lowrey L., Pedersen P.A., Gourdon P., Gotfryd K.

2018 - Vascular Endothelial Growth Factor (VEGF) Induced Downstream Responses to Transient Receptor Potential Vanilloid 1 (TRPV1) and 3-Iodothyronamine (3-T1AM) in Human Corneal Keratocytes

icon pap   Port-a-Patch publication in Frontiers in Pharmacology (2018)

Türker, E., Garreis, F., Khajavi, N., Reinach, P.S., Joshi, P., Brockmann, T., Lucius, A., Ljubojevic, N., Turan, E., Cooper, D., Schick, F., Reinholz, R., Pleyer, U., Köhrle, J., Mergler, S.

2018 - TRPM8 Activation via 3-Iodothyronamine Blunts VEGF-Induced Transactivation of TRPV1 in Human Uveal Melanoma Cells

icon pap   Port-a-Patch publication in Frontiers in Pharmacology (2018)

Walcher, L., Budde, C., Böhm, A., Reinach, P.S., Dhandapani, P., Ljubojevic, N., Schweiger, M.W., von der Waydbrink, H., Reimers, I., Köhrle, J., Mergler, S.

2018 - 3-Iodothyronamine Activates a Set of Membrane Proteins in Murine Hypothalamic Cell Lines

icon pap   Port-a-Patch publication in Frontiers in Endocrinology (2018)

Bräunig J., Mergler S., Jyrch S., Hoefig C.S., Rosowski M., Mittag J., Biebermann H., Khajavi N.

2014 - Calcium regulation by temperature-sensitive transient receptor potential channels in human uveal melanoma cells

icon pap  Port-a-Patch publication in Cellular Signalling (2014)

Mergler S., Derckx R., Reinach P.S., Garreis F., Böhm A., Schmelzer L., Skosyrski S., Ramesh N., Abdelmessih S., Polat O.K., Khajavi N., Riechardt A.I.

2013 - Functional significance of thermosensitive transient receptor potential melastatin channel 8 (TRPM8) expression in immortalized human corneal endothelial cells

icon pap  Port-a-Patch publication in Experimental Eye Research (2013)

Mergler S., Mertens C., Valtink M., Reinach P.S., Castelo Székely V., Slavi N., Abdelmessih S., Türker E., Fels G., Pleyer U.

selectively permeable

We use cookies on our website. Some of them are essential for the operation of the site, while others help us to improve this site and the user experience (tracking cookies). You can decide for yourself whether you want to allow cookies or not. Please note that if you reject them, you may not be able to use all the functionalities of the site.