TRPV3 | Transient Receptor Potential Cation Channel Subfamily V Member 3
Family:
Transient receptor potential channels
Subgroups:
TRPC (TRPC1–TRPC7), TRPV (TRPV1-TRPV6), TRPA1, TRPM (TRPM1–TRPM8), TRPP (TRPP1–TRPP3, PKD1, PKDREJ, PKDL1–PKDL3), TRPML (TRPML1–TRPML3), TRPN
Topology:
Most TRP channels are composed of 6 transmembrane domains (helices) with intracellular N- and C-termini, non-selectively permeable to various cations
TRPV3: Background information
Gene:
SCN10A
Human Protein:
UniProt Q9Y5Y9
Tissue:
Brain (small diameter DRG neurons)
Function/ Application:
Excitability of neurons, nociceptive transmission
Pathology:
Neuropathic pain, Multiple Sclerosis
Accessory subunits:
b1, b2, b3
Interaction:
p11, PDZD2 and syntrophin-associated serine/threonine kinase (SASTK), Contactin, CAP-1A
Modulator:
Tetrodotoxin, lidocaine, benzocaine, A-887826, A-803467
Assays:
Patch Clamp: whole cell, room temperature, State- and use-dependence
Particularities:
Cell systems:
hNaV1.8 stably expressing CHO cell line (Charles River); rNaV1.8 stably expressed in ND7/23 cell line (EMD Millipore)
Recommended Reviews:International Union of Pharmacology. XLVII. Nomenclature and Structure-Function Relationships of Voltage-Gated Sodium Channels. Pharmacol Rev 57: 397–409, Catterall, et al. 2005
Data and Applications
TRPV3 - Temperature Activation
Patchliner data and applications:
Cells were kindly supplied by Millipore.
Recordings from a HEK cell expressing TRPV3 were made with the Patchliner showing activation by heat. External solution was heated inside the Patchliner pipette to the temperature shown and applied to the cells. TRPV3 was activated at temperatures ≥ 38°C.
Posters
2016 - Next level toxicity screening: From single channel to overall cell behavior
Orbit mini, 
CardioExcyte 96 and 
SyncroPatch 384PE poster, Meeting of the French Society of Toxinology (SFET) 2015 
(0.9 MB)
2015 - The backstage pass to study your favorite TRP channel
Port-a-Patch and Patchliner and SyncroPatch 384PE and SyncroPatch 384PE poster, TRP Meeting 2015 (2.2 MB)
Application Notes
TRPV3 - "Heat activation of TRPV3 on Nanion's Patchliner"
Patchliner application note: (0.6 MB)
Publications
2015 - Novel screening techniques for ion channel targeting drugs
Patchliner, SyncroPatch 384PE and CardioExcyte 96 publication in Channels (2015)
Authors:
Obergrussberger A., Stölzle-Feix S., Becker N., Brüggemann A., Fertig N., Möller C.
2015 - Different Ligands of the TRPV3 Cation Channel Cause Distinct Conformational Changes As Revealed by Intrinsic Tryptophan Fluorescence Quenching
Port-a-Patch and 
Vesicle Prep Pro publication in Journal of Biological Chemistry (2015)
Authors:
Billen B., Brams M., Debaveye S., Remeeva A., Alpizar Y.A., Waelkens E., Kreir M., Brüggemann A., Talavera K., Nilius B., Voets T., Ulens C.
2011 - Thermosensitive transient receptor potential channels in human corneal epithelial cells
Port-a-Patch publication in Journal of Cellular Physiology (2011)
Authors:
Mergler S., Garreis F., Sahlmüller M., Reinach P.S., Paulsen F., and Pleyer U.
2011 - State-of-the-art automated patch clamp devices: heat activation, action potentials, and high throughput in ion channel screening
Port-a-Patch, Patchliner and SyncroPatch 96 (a predecessor model of SyncroPatch 384PE) publication in Frontiers in Pharmacology (2011)
Authors:
Stoelzle S., Obergrussberger A., Brüggemann A., Haarmann C., George M., Kettenhofen R., Fertig N.
2010 - TRPV channels mediate temperature-sensing in human corneal endothelial cells
Port-a-Patch publication in Experimental Eye Research (2010)
Authors:
Mergler S., Valtink M., Coulson-Thomas V.J., Lindemann D., Reinach P.S., Engelmann K., Pleyer U.