TRPV3 | Transient Receptor Potential Cation Channel Subfamily V Member 3
Family:
Transient receptor potential channels
Subgroups:
TRPC (TRPC1–TRPC7), TRPV (TRPV1-TRPV6), TRPA1, TRPM (TRPM1–TRPM8), TRPP (TRPP1–TRPP3, PKD1, PKDREJ, PKDL1–PKDL3), TRPML (TRPML1–TRPML3), TRPN
Topology:
Most TRP channels are composed of 6 transmembrane domains (helices) with intracellular N- and C-termini, non-selectively permeable to various cations
TRPV3: Background information
TRPV3 is a putative receptor-activated non-selective calcium permeant cation channel. It is activated by innocuous (22°C - 40°C) temperatures and shows an increased response at noxious temperatures greater than 39 degrees Celsius. Activation exhibits an outward rectification.
Gene:
TRPV3
Human Protein:
UniProt Q8NET8
Tissue:
Widely expressed in the human body, abundantly expressed in the CNS, keratinocyte layers of the outer root sheath and matrix of the hair follicles
Function/ Application:
TRPV3 plays a role in a variety of processes, including temperature sensation and vasoregulation. TRPV3 is a negative regulator of hair growth and cycling: TRPV3-coupled signaling suppresses keratinocyte proliferation in hair follicles and induces apoptosis and premature hair follicle regression (catagen)
Pathology:
Olmsted syndrome (OLMS), Palmoplantar keratoderma, non-epidermolytic, focal 2 (FNEPPK2).
Selective TRPV3 inhibition is being investigated for therapeutic potential for treatment of chronic pruritus, skin allergy, or inflammation-related skin diseases.
Interaction:
TRPV3 may form a heteromeric channel with TRPV1, and it interacts with Calmodulin, epidermal growth factor receptor, AKAP79 (AKAP-5)
Modulator:
Carvacrol, thymol, eugenol, camphor, 2-APB, isopentenyl diphosphate, aspirin-triggered resolvin D1, forsythoside B, isopentenyl diphosphate, osthole, ruthenium red
Assays:
Patch Clamp: whole cell, temperature control
Recommended Reviews:International Union of Pharmacology. XLIII. Compendium of voltage-gated ion channels: transient receptor potential channels., Pharmacol Rev 55(4):591-6 Clapham, et al. 2003
Data and Applications
TRPV3 - Temperature Activation
Patchliner data and applications:
Cells were kindly supplied by Millipore.
Recordings from a HEK cell expressing TRPV3 were made with the Patchliner showing activation by heat. External solution was heated inside the Patchliner pipette to the temperature shown and applied to the cells. TRPV3 was activated at temperatures ≥ 38°C.
Posters
2021 - Activation and inhibition of assay-ready TRPA1 and TRPV cells: an automated patch clamp study
Port-a-Patch, 
Patchliner and 
SyncroPatch 384i (a predecessor model of SyncroPatch 384) Biophysical Society Meeting 2021 
(1.5MB)
2016 - Next level toxicity screening: From single channel to overall cell behavior
Orbit mini, 
CardioExcyte 96 and 
SyncroPatch 384PE (a predecessor model of the SyncroPatch 384) poster, Meeting of the French Society of Toxinology (SFET) 2015 (0.9 MB)
2015 - The backstage pass to study your favorite TRP channel
Port-a-Patch and Patchliner and SyncroPatch 384PE (a predecessor model of the SyncroPatch 384) poster, TRP Meeting 2015 (2.2 MB)
Application Notes
TRPV3 - "Heat activation of TRPV3 on Nanion's Patchliner"
Patchliner application note: (0.6 MB)
Publications
2019 - Purification of Functional Human TRP Channels Recombinantly Produced in Yeast
Orbit mini publication in Cells (2019)
Authors:
Zhang L., Wang K., Klaerke D.A., Calloe K., Lowrey L., Pedersen P.A., Gourdon P., Gotfryd K.
2015 - Novel screening techniques for ion channel targeting drugs
Patchliner, SyncroPatch 384PE (a predecessor model of SyncroPatch 384i) and CardioExcyte 96 publication in Channels (2015)
Authors:
Obergrussberger A., Stölzle-Feix S., Becker N., Brüggemann A., Fertig N., Möller C.
2015 - Different Ligands of the TRPV3 Cation Channel Cause Distinct Conformational Changes As Revealed by Intrinsic Tryptophan Fluorescence Quenching
Port-a-Patch and 
Vesicle Prep Pro publication in Journal of Biological Chemistry (2015)
Authors:
Billen B., Brams M., Debaveye S., Remeeva A., Alpizar Y.A., Waelkens E., Kreir M., Brüggemann A., Talavera K., Nilius B., Voets T., Ulens C.
2011 - Thermosensitive transient receptor potential channels in human corneal epithelial cells
Port-a-Patch publication in Journal of Cellular Physiology (2011)
Authors:
Mergler S., Garreis F., Sahlmüller M., Reinach P.S., Paulsen F., and Pleyer U.
2011 - State-of-the-art automated patch clamp devices: heat activation, action potentials, and high throughput in ion channel screening
Port-a-Patch, Patchliner and SyncroPatch 96 (a predecessor model of SyncroPatch 384PE) publication in Frontiers in Pharmacology (2011)
Authors:
Stoelzle S., Obergrussberger A., Brüggemann A., Haarmann C., George M., Kettenhofen R., Fertig N.
2010 - TRPV channels mediate temperature-sensing in human corneal endothelial cells
Port-a-Patch publication in Experimental Eye Research (2010)
Authors:
Mergler S., Valtink M., Coulson-Thomas V.J., Lindemann D., Reinach P.S., Engelmann K., Pleyer U.