• TRPV4

    Hypotonicity-induced TRPV4 channel activation, measured on the Port-a-Patch

    Mergler et al. (2011)

TRPV4 | Transient Receptor Potential Cation Channel Subfamily V Member 4

Family:
Transient  receptor potential channels

Subgroups:
TRPC (TRPC1–TRPC7), TRPV (TRPV1-TRPV6), TRPA1, TRPM (TRPM1–TRPM8), TRPP (TRPP1–TRPP3, PKD1, PKDREJ, PKDL1–PKDL3), TRPML (TRPML1–TRPML3), TRPN

Topology:
Most TRP channels are composed of 6 transmembrane domains (helices) with intracellular N- and C-termini, non-selectively permeable to various cations

TRPV4: Background information

Gene:
SCN10A

Human Protein:
UniProt Q9Y5Y9

Tissue:
Brain (small diameter DRG neurons)

Function/ Application:
Excitability of neurons, nociceptive transmission

Pathology:
Neuropathic pain, Multiple Sclerosis

Accessory subunits:
b1, b2, b3

Interaction:
p11, PDZD2 and syntrophin-associated serine/threonine kinase (SASTK), Contactin, CAP-1A

Modulator:
Tetrodotoxin, lidocaine, benzocaine, A-887826, A-803467

Assays:
Patch Clamp: whole cell, room temperature, State- and use-dependence

Particularities:

Cell systems:
hNaV1.8 stably expressing CHO cell line (Charles River); rNaV1.8 stably expressed in ND7/23 cell line (EMD Millipore)

Recommended Reviews:
International Union of Pharmacology. XLVII. Nomenclature and Structure-Function Relationships of Voltage-Gated Sodium Channels. Pharmacol Rev 57: 397–409, Catterall, et al. 2005 

Publications

2018 - Electrophysiological experiments in microgravity: lessons learned and future challenges

icon pap   Port-a-Patch publication in Microgravity (2018)

Authors:
Wuest S.L., Gantenbein B., Ille F.,Egli M.

2018 - Differential interactions of bacterial lipopolysaccharides with lipid membranes: implications for TRPA1-mediated chemosensation

icon vpp   Vesicle Prep Pro publication in Scientific Reports (2018)

Authors:
Startek J.B., Talavera K., Voets T., Alpizar Y.A.

2012 - Calcium regulation by thermo- and osmosensing transient receptor potential vanilloid channels (TRPVs) in human conjunctival epithelial cells

icon pap   Port-a-Patch publication in Histochemistry and Cell Biology (2012)

Authors: 
Mergler S., Garreis F., Sahlmüller M., Lyras E.-M., Reinach P.S., Dwarakanath A., Paulsen F., Pleyer U.

2011 - Thermosensitive transient receptor potential channels in human corneal epithelial cells

icon pap   Port-a-Patch publication in Journal of Cellular Physiology (2011)

Authors: 
Mergler S., Garreis F., Sahlmüller M., Reinach P.S., Paulsen F., and Pleyer U.

2011 - Characterization of transient receptor potential vanilloid channel 4 (TRPV4) in human corneal endothelial cells

icon pap   Port-a-Patch publication in Experimental Eye Research (2011)

Authors: 
Mergler S., Valtink M., Taetz K., Sahlmüller M., Fels G., Reinach P.S., Engelmann K., Pleyer U.

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