Hypotonicity-induced TRPV4 channel activation, measured on the Port-a-Patch

    Mergler et al. (2011)

TRPV4 | Transient Receptor Potential Cation Channel Subfamily V Member 4

Transient  receptor potential channels


Most TRP channels are composed of 6 transmembrane domains (helices) with intracellular N- and C-termini, non-selectively permeable to various cations

TRPV4: Background information

TRPV4 is a non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity exhibiting an outward rectification. It is activated by exposure to hypotonicity within the physiological range as well as by heat, low pH, citrate and phorbol esters. An increase of intracellular Ca2+ potentiates currents. The channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism.


Human Protein:
UniProt Q9HBA0

Trachea, kidney, liver, spleen, skin, brain, PNS, heart

Function/ Application:
TRPV4 has been found to be involved in multiple phsiological functions, as the regulation of systemic osmotic pressure in the brain, the skin barrier, pain, osmosensation, homeostasis. In the vascular endothelium, it contributes to intracellular Ca2+ homeostasis and regulation of cell volume while responding to mechanical stresses on the vasculature. It is crucially for the function of the cardiac, respiratory, urinary, skeletal, and digestive systems, as well as in the central and peripheral nervous systems.

Brachyolmia 3 (BCYM3), Spondylometaphyseal dysplasia Kozlowski type (SMDK), Metatropic dysplasia (MTD), Neuronopathy, distal hereditary motor, 8 (HMN8), Charcot-Marie-Tooth disease 2C (CMT2C), Scapuloperoneal spinal muscular atrophy (SPSMA), Spondyloepiphyseal dysplasia Maroteaux type (SEDM), Parastremmatic dwarfism (PSTD), Digital arthropathy-brachydactyly, familial (FDAB), Avascular necrosis of the femoral head, primary 2 (ANFH2)

Calmodulin, Map7, Src family Tyr protein kinases (LYN, SRC, FYN, HCK, LCK and YES), CTNNB1, PACSIN1, PACSIN2, PACSIN3, ITPR3, AQP5, ANO1, PKD2, and is part of the complex containing containing MLC1, AQP4, HEPACAM and ATP1B1.

Cannabidiol, Resiniferatoxin, GSK1016790A, RN1747, 4α-PDD, GSK2193874, HC067047, La3+, Gd3+, ruthenium red

Patch Clamp: whole cell, temperature control

Recommended Reviews:
International Union of Pharmacology. XLIII. Compendium of voltage-gated ion channels: transient receptor potential channels., Pharmacol Rev 55(4):591-6 Clapham, et al. 2003
TRPV4: Molecular Conductor of a Diverse Orchestra, Physiological Reviews 96(3):911-973 John P.M White et al. 2016

Application Notes


2022- The discovery of (1R, 3R)-1-(3-chloro-5-fluorophenyl)-3-(hydroxymethyl)-1,2,3,4-tetrahydroisoquinoline-6-carbonitrile, a potent and selective agonist of human transient receptor potential cation channel subfamily m member 5 (TRPM5) and evaluation of as a potential gastrointestinal prokinetic agent

 icon sp96 SyncroPatch 384 publication in Bioorganic & Medicinal Chemistry (2022)

Davies M.R., Martinec M., Walls R., Schwarz R., Mirams G.R., Wang K., Steiner G., Surinach A., Flores C., Lave T., Singer T., Polonchuk L.

2021 - Glucosylsphingosine evokes pruritus via activation of 5-HT2A receptor and TRPV4 in sensory neurons

icon pap   Port-a-Patch Publication in British Journal of Pharmacology (2021)

Sanjel B., Kim B-H., Song M-H., Carstens E., Shim W-S.

2019 - Purification of Functional Human TRP Channels Recombinantly Produced in Yeast

Icon Orbit Mini   Orbit mini publication in Cells (2019)

Zhang L., Wang K., Klaerke D.A., Calloe K., Lowrey L., Pedersen P.A., Gourdon P., Gotfryd K.

2018 - Electrophysiological experiments in microgravity: lessons learned and future challenges

icon pap   Port-a-Patch publication in Microgravity (2018)

Wuest S.L., Gantenbein B., Ille F.,Egli M.

2018 - Differential interactions of bacterial lipopolysaccharides with lipid membranes: implications for TRPA1-mediated chemosensation

icon vpp   Vesicle Prep Pro publication in Scientific Reports (2018)

Startek J.B., Talavera K., Voets T., Alpizar Y.A.

2012 - Calcium regulation by thermo- and osmosensing transient receptor potential vanilloid channels (TRPVs) in human conjunctival epithelial cells

icon pap   Port-a-Patch publication in Histochemistry and Cell Biology (2012)

Mergler S., Garreis F., Sahlmüller M., Lyras E.-M., Reinach P.S., Dwarakanath A., Paulsen F., Pleyer U.

2011 - Thermosensitive transient receptor potential channels in human corneal epithelial cells

icon pap   Port-a-Patch publication in Journal of Cellular Physiology (2011)

Mergler S., Garreis F., Sahlmüller M., Reinach P.S., Paulsen F., and Pleyer U.

2011 - Characterization of transient receptor potential vanilloid channel 4 (TRPV4) in human corneal endothelial cells

icon pap   Port-a-Patch publication in Experimental Eye Research (2011)

Mergler S., Valtink M., Taetz K., Sahlmüller M., Fels G., Reinach P.S., Engelmann K., Pleyer U.

2008 - Production of a specific extracellular inhibitor of TRPM3 channels

icon pl  Patchliner publication in British Journal of Pharmacology (2008)

Naylor J., Milligan C.J., Zeng F., Jones C., Beech D.J.

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