• VDAC's

    Representative parallel recording from 3 VDAC channel measured on the Orbit mini

VDAC1 - 3 | Voltage-dependent Anion-selective Channel Protein 1 - 3

Family:
Voltage-dependent Anion Channels

Subgroups:
3 members: VDAC1, VDAC2, VDAC3

Topology:
The channel protein froms a beta barrel which spans the membrane

Function:
Forms a channel through the mitochondrial outer plasma membrane, and also plasma membrane, involved in cell volume regulation and apoptosis 

Application Notes

Data and Applications

VDAC - Application of Fluoxetine

Icon Orbit Mini   Orbit Mini vdac 1Orbit mini and applications:
Data were kindly provided by Ionera.

Current recordings of single VDA channels from selected bilayers in parallel.
VDAC was reconstituted in DPhPC membranes and partially blocked upon the addition of Fluoxetine (Prozac).

(A) Representative parallel recording from 3 VDAC channels in DPhPC membranes. Conditions: 1 M KCl, 25 mM Tris, pH 7,5, voltage change protocol is given below.
(B) Current trace illustration partial blocade of VDAC by Fluoxetine (Prozac).
(C) Current-voltage trace of a single VADC channel under a ramping voltage of -70/+70 mV before (green) and after (blue) addition of 50 µm Fluoxetine

Purified VDAC sample is a generous gift from Prof. Stephan Nussberger to Ionera, Department Biophysics, University of Stuttgart, Germany

Publications

2017 - Discovery of benzimidazole derivatives as modulators of mitochondrial function: A potential treatment for Alzheimer's disease

icon pl  Patchliner publication in PLoS ONE (2017)

Authors: 
Kim T., Yang H.Y., Park B.G., Jung S.Y., Park J.H., Park K.D., Min S.J., Tae J., Yang H., Cho S., Cho S.J., Song H., Mook-Jung I., Lee J., Pae A.N.

2016 - Voltage Dependence of Conformational Dynamics and Subconducting States of VDAC-1

icon pap  Port-a-Patch publication in Biophysical Journal (2016)

Authors: 
Briones R., Weichbrodt C., Paltrinieri L., Mey I., Villinger S., Giller K., Lange A., Zweckstetter M., Griesinger C., Becker S., Steinem C., de Groot B.L.

2016 - pH-sensitive K+ channel TREK-1 is a novel target in pancreatic cancer

icon sp96  SyncroPatch 384PE publication in Biochimica et Biophysica Acta (2016)

Authors: 
Sauter D.R.P., Sørensen C.E., Rapedius M., Brüggemann A., Novak I.

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