Collaborative Activities with Cellular Dynamics

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NCX1 - Current traces recorded from Cor.4U Cardiomyocytes, iCell Cardiomyocytes and HEK cells

NCX1 Cor4U icell HEK

Icon N1   SURFE2R N1 data and applications:

Cardiac NCX1 was activated using 30 μM Ca2+ on the SURFE2R N1. NCX1 was recorded from Cor.4U® Cardiomyocytes, iCell® Cardiomyocytes2 or overexpressed in HEK cells. The calcium affinity was determined for NCX1 recorded from iCell® Cardiomyocytes2 and HEK cells, shown on the right.  

 

NCX1 - Block by KB-R7943

NCX1 KBR7943 iCell Cor4U HEK

Icon N1   SURFE2R N1 data and applications:

Cardiac NCX1 expressed in iCell® Cardiomyocytes2, Cor.4U® Cardiomyocytes or HEK cells was blocked by increasing concentrations of KB-R7943.  

 

NCX1 - Block by SEA0400

NCX1 iCell HEK SEA0400

Icon N1   SURFE2R N1 data and applications:

Cardiac NCX1 expressed in iCell® Cardiomyocytesor HEK cells was blocked by increasing concentrations of SEA0400, a specific blocker of NCX.  

 

2018 - Expression and pharmacology of GluA2-containing AMPA receptors in cell lines and stem cell-derived neurons

icon pap   Port-a-Patch,   icon pl   Patchliner and   icon sp96   SyncroPatch 384PE poster, Europhysiology Meeting 2018  logo pdf   (0.9 MB)

2018 - Combining electrophysiology and contractility recordings for more complete assessment of hiPSC-CMs

icon sp96   SyncroPatch 384PE,   icon pl   Patchliner and   Icon CardioExcyte 96 simpel RGB   CardioExcyte 96 poster, Europhysiology Meeting 2018  logo pdf   (1.4 MB)

2018 - A Sensor Based Technique for Pharmacological Safety Testing of Cardiac Transport Proteins NCX, NaKATPase and Respiratory Chain Complexes

Icon CE   CardioExcyte 96 and   Icon 96SE   SUFE²R 96SE poster, SPS 2018  logo pdf   (1.7 MB)

2018 - Investigating pain pathways by inhibition of voltage-gated sodium channels

icon sp96   SyncroPatch 384PE and   icon pl   Patchliner poster, FENS Meeting 2018  logo pdf   (2.5 MB)

2017 - Cardiomyocytes in Voltage Clamp and Current Clamp by Automated Patch Clamp

icon sp96   SyncroPatch 384PE and   icon pl   Patchliner poster, BPS Meeting 2017  logo pdf   (1.7 MB)

2018 - Cross-site comparison of excitation-contraction coupling using impedance and field potential recordings in hiPSC cardiomyocytes

Icon CE   CardioExcyte 96 publication in Journal of Pharmacological and Toxicological Methods (2018)

Authors:
Bot C.T., Juhasz K., Haeusermann F., Polonchuk L., Traebert M., Stölzle-Feix S.

Cardiac Ion Channels - Pharmacology of Vandetanib

CiPA PE CE Pharmacology VandetanibIcon CE    CardioExcyte 96 and   icon pl   Patchliner data and applications:
Cells were kindly provided by Charles River and Cellular Dynamics.

The image on the left hand side displays the results of the blocking effect of Vandetanib on hERG, NaV1.5, CaV1.2 and KV4.3. The compound induced arrhythmia when iPSC-CM were exposed to a minimum concentration of 1 µM. Arrhytmic events were both detected in field potential recordings as well as in the impedance based contractility.

Cardiac Ion Channels - Pharmacology of Sotalol

CiPA PE CE Pharmacology SotalolIcon CE    CardioExcyte 96 and   icon sp96   SyncroPatch 384PE data and applications:
Cells were kindly provided by Charles River and Cellular Dynamics.

The image on the left hand side displays the results of the blocking effect of Sotalol on hERG. The result is in good agreement with manual patch clamp data (Crumb et al., 2016). The compound induced arrhythmia when iPSC-CM were exposed to a minimum concentration of 10 µM. Arrhytmic events were both detected in field potential recordings as well as in the impedance based contractility measurements.

2017 - Cross-site comparison of myocyte phase II compounds on different iPS cardiomyocytes based on CardioExcyte 96 recordings

Icon CE   CardioExcyte 96 presentation (slide deck)   logo pdf   (2.2 MB)

2017 - HTS in Cardiac Safety

Icon CE   CardioExcyte 96 presentation (slide deck)   logo pdf   (4.0 MB)

Cardiomyocytes - "Impedance and EFP recordings of iCell Cardiomyocytes² on the CardioExcyte 96"

Icon CE   CardioExcyte 96 Application Note   logo pdf   (2.8 MB)
Cells were kindly provided by Cellular Dynamics.

2016 - Automated Patch Clamp Meets High-Throughput Screening: 384 Cells Recorded in Parallel on a Planar Patch Clamp Module

icon sp96  SyncroPatch 384PE publication in Journal of Lab Automation (2016)

Authors: 
Obergrussberger A., Brüggemann A., Goetze T.A., Rapedius M., Haarmann C., Rinke I., Becker N., Oka T., Ohtsuki A., Stengel T., Vogel M., Steindl J., Mueller M., Stiehler J., George M., Fertig N.

2015 - Novel screening techniques for ion channel targeting drugs

icon pl  Patchliner,   icon sp96   SyncroPatch 384PE and   Icon CE   CardioExcyte 96 publication in Channels (2015)

Authors: 
Obergrussberger A., Stölzle-Feix S., Becker N., Brüggemann A., Fertig N., Möller C.

2012 - Characterizing Human Ion Channels in Induced Pluripotent Stem Cell-Derived Neurons

icon pl   Patchliner publication in Journal of Biomolecular Screening (2012)

Authors: 
Haythornthwaite A, Stoelzle S, Hasler A, Kiss A, Mosbacher J, George M, Brüggemann A, Fertig N.

2013 - Minimized cell usage for stem cell-derived and primary cells on an automated patchclamp system

icon pl  Patchliner publication in Journal of Pharmacological and Toxicological Methods (2013)

Authors: 
Becker N., Stoelzle S., Göpel S., Guinot D., Mumm P., Haarmann C., Malan D., Bohlen H., Kossolov E., Kettenhofen R., George M., Fertig N., Brüggemann A.

2015 - New Easy-to-Use Hybrid System for Extracellular Potential and Impedance Recordings.

Icon CE  CardioExcyte 96 publication in Journal of Laboratory Automation (2015)

Authors: 
Doerr L., Thomas U., Guinot D.R., Bot C.T., Stoelzle-Feix S., Beckler M., George M., Fertig N.

GABAA Receptor - Currents in iCell Neurons

GABA Bicuculline neurons

icon pl   Patchliner data and applications:
Cells were kindly provided by Cellular Dynamics.

Activation of GABAA receptor currents by 30 μM GABA and partial block of the current response by 1 μM bicuculline. Bicuculline was pre-applied for at least 30 s before co-application with GABA (30 μM). Approximately 50% of the current was blocked by 1 μM bicuculline.

Neurons (iPSC-derived) - Voltage Gated Potassium Currents

KvIV Neurons Figure3 icon pl   Patchliner data and applications:
Cells were kindly provided by Cellular Dynamics.

A Voltage-gated K+ current recorded in iCell® neurons. Current responses to a voltage step protocol. An outward K+ current can be seen in this cell.
B Corresponding IV plot from an average of 7 cells. 

Neurons (iPSC-derived) - TXX Block of Sodium Currents

TTX Neurons Figure2icon pl   Patchliner data and applications:
Cells were kindly provided by Cellular Dynamics.

A Block of Na+ current by increasing concentrations of TTX. Current was blocked by low nM concentrations of TTX indicating a TTX sensitive Na+ channel type expressed in this cell.
B Concentration response curve for TTX inhibition, IC50 = 4.9 nM (n =1).

Neurons (iPSC-derived) - Voltage-Gated Sodium Currents

NaIV Neurons Figure1icon pl   Patchliner data and applications:
Cells were kindly provided by Cellular Dynamics.

Voltage-gated Na+ current recorded in iCell® Neurons. Current responses to a voltage step protocol.
A large inward Na+ current can be seen in this cell with a K+ outward current present at positive voltages.
B Normalised IV plot from an average of 4 cells.

Neurons - "Stem cell-derived neurons recorded on Nanion's Patchliner"

icon pl   Patchliner application note:   logo pdf   (0.4 MB)
Cells were kindly provided by Cellular Dynamics.

NaV1.5 - Analyzing iPSC-derived Cardiomyocytes

0NavLidocaineScreenicon sp96   SyncroPatch 96 (a predecessor model of SyncroPatch 384PE) data and applications:
Cells were kindly supplied by Cellular Dynamics.

Inhibition of NaV1.5 currents in stem cell-derived cardiomyocytes (iCells) by lidocaine. Lidocaine concentrations: 6 µM, 62 µM and 620 µM. The obatined  IC50 -value was 14 µM.

 

Cardiac Action Potentials - Automated recordings from iCells

0AP Patchliner CDI

icon pl   Patchliner data and applications:
The stem cell-derived cardiomyocytes (iCell) were kindly supplied by Cellular Dynamics.

In this example both Na+ and Ca2+ mediate the action potential. When nifedipine is applied in the current clamp mode, the action potential is shortened significantly due to block of the calcium channels.

 

Cardiomyocytes - Data from different cell providers

Icon CE   CardioExcyte different ProvidersCardioExcyte data and applications:
Cells were kindly provided by Axiogenesis, Cellular Dynamics, GE Healthcare, Pluriomics, ReproCell, Takara Bio Cellartis Clonetech.

The CardioExcyte 96 allows for
• Non-invasive, label-free measurements of beating cardiomyocyte networks
• 96 recording wells in parallel with 1 ms time resolution
• Quick experiments or long-term compound effects on cardiotoxicity
• Real-time access to beating parameters
• Outstanding software for data analysis and export
• Cost effcient consumables - 96-well format

Cardiomyocytes - Effect of E4031 on the impedance and EFP signals on iCell cardiomyocytes

Icon CE   CardioExcyte E3031CardioExcyte data and applications:
Cells were kindly provided by Cellular Dynamics.

Effect of the specific hERG blocker, E4031, on the impedance and EFP signals. A Impedance signal of 12 wells in control conditions (left) and the same 12 wells after 13 mins incubation in E4031 at the concentrations indicated (right). E4031 (300 nM, 3 mins incubation) induces EAD, shown in the inset, which can lead to potentially fatal ventricular arrhythmias. B EFP signal of 12 wells in control conditions (left) and the same 12 wells after 13 mins incubation in E4031 at the concentrations indicated (right). E4031(300 nM, 30 mins incubation) also causes arrhythmic effects in the EFP mode shown in the inset.

Cardiomyocytes - Effect of the ß-adrenergic receptor agonist, Isoproterenol, on the impedance and EFP signals on iCells

Icon CE   CardioExcyte IsoproterenolCardioExcyte data and applications:
Cells were kindly provided by Cellular Dynamics.

A Impedance signal of 12 wells in control conditions (left) and the same 12 wells after 13 mins incubation in isoproterenol at the concentrations indicated. Isoproterenol acts as a heart stimulant, it increases heart rate, an effect which is highlighted in the inset where the trace in the presence of isoproterenol (300 nM; black) and the control trace (red) are shown overlaid. In the presence of even low concentrations of isoproterenol (1 nM), an increase in the beat rate is observed. B Bar graph showing beat rate before (dark blue) and after (light blue) incubation in isoproterenol at the concentrations indicated. At all concentrations, a clear increase in beat rate is observed. C EFP signal of 12 wells in control conditions (left) and the same 12 wells after 30 mins incubation in isoproterenol at the concentrations indicated (right). Isoproterenol shortens the FPD, an effect which is highlighted in the inset where the trace in the presence of isoproterenol (300 nM; black) and the control trace (red) are shown overlaid.

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