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2016 - Next level toxicity screening: From single channel to overall cell behavior

Icon Orbit Mini   Orbit mini,   Icon CE   CardioExcyte 96 and   icon sp96   SyncroPatch 384PE (a predecessor model of the SyncroPatch 384) poster, Meeting of the French Society of Toxinology (SFET) 2015  logo pdf   (0.9 MB)


Many lead compounds fail in the late stages of the drug development process mainly by inflicting drug induced injury on liver, heart or other organs. Therefore, devices for detecting possible cell toxicity in early stages of the development process are highly demanded. Especially ion channels represent an important class of drug targets for in vitro pharmacological profiling. High throughput screening (HTS) assays such as automated electrophysiological patch clamp and impedance based assays allow for the determination of drug effects on a whole cell level whereas artificial bilayers provide a robust environment for the assessment of single ion channel molecules. We here present the CardioExcyte 96, a system providing a combination of Electric Impedance Spectroscopy (EIS) as well as Electric Field Potential (EFP) readout for a network of diverse cells like iPS cardiomyocytes or hepatocyte-l ike cells which is exemplified by toxicity effects util izing reference compounds such as Dofetilide (on iPS cardiomyocytes) or Paracetamol (on hepatocyte-like cells). Furthermore we present the temperature dependent activation or deactivation of
different Transient Receptor Potential (TRP) channels by means of planar patch clamping on our HTS platforms Patchliner and SyncroPatch 384PE as well as with
highest resolution on a single channel level on our recently introduced Orbit mini setup.

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