Patchliner application note: (0.4 MB)
Cells were kindly provided by Cellular Dynamics.
Cellular Dynamics International is developing iCell® Neurons, human iPS cell-derived neurons. These neurons have been used on Nanion’s Patchliner, an automated patch clamp device for recording from up to 8 cells simultaneously. Cells were thawed and cultured as per the manufacturer’s instructions. After plating, cells developed neuronal outgrowth within 24 hours and could be kept in culture for 2 weeks. An investigation into voltage- and ligand-gated ion channels expressed in these cells was undertaken using the Patchliner. The first experiments are shown here and offer promising results for combining a cellular model for neurons and an increased throughput patch clamp device.