Collaborative Activities with acCELLerate

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acCELLerate is a specialist for large-scale production and cryopreservation of highly functional assay ready cells. The instant use of cells from a frozen stock became a widely accepted approach preferred to continuously passaged cells. Assay ready cells are used manifold for the screening of new drugs, the animal free safety testing, and for the potency testing of biotherapeutics in clinical development and manufacturing control. Assay ready cells increase the flexibility of any cell-based project and can significantly improve assay precision.

Based on 16 years of experience acCELLerate has developed freezing protocols and established well-controlled processes to prepare assay ready Frozen Instant Cells of high quality and robustness. Frozen Instant Cells can be used like a reagent without prior cultivation. acCELLerate provides assay ready cells from multiple cell lines, validated instaCELL bioassay kits, as well as custom cell banks of Frozen Instant Cells with comprehensive documentation to meet specific requirements of the different fields of application.

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TRPA1 - Activation by carvacrol

TRPA1 Carvacrol SP384iicon sp96   SyncroPatch 384i data and applications: 
Cells were kindly provided by AcCELLerate.

Activation of TRPA1 expressed in CHO cell on the SyncroPatch 384i. A Screenshot of the PatchControl 384 software depicts raw data traces of TRPA1- expressing CHO cells as recorded on one NPC-384 patch clamp chip (4 holes). A single concentration of carvacrol was added to each well and the concentration response curve calculated across the plate. B Average CRC (left) for carvacrol for n = 355 wells and average traces (right). TRPA1 was robustly activated by carvacrol. At higher concentrations (above 400 µM) desensitization of the channel was observed (tachyphylaxis) which resulted in smaller amplitudes upon repeated application.

TRPA1 - Inhibition by A967079 and AMG0902

TRPA1 Inhibition SP384icon sp96   SyncroPatch 384i data and applications: 
Cells were kindly provided by AcCELLerate.

Inhibition of TRPA1 by A967079 and AMG0902. A TRPA1-mediated responses were blocked by A967079 in a concentration-dependent manner with an IC50 value (12.4 nM) in good agreement with the literature value of 50 nM. B TRPA1-mediated responses were blocked by AMG0902 in a concentration-dependent manner with an IC50 value of 48 nM (n = 349) in good agreement with the literature value of 68 nM.

 

Qualification of Patch Ready Cells on a SyncroPatch 384PE

icon sp96   SyncroPatch 384PE (a predecessor model of SyncroPatch 384i) application note:   logo pdf   (1.0 MB)
Patch Ready Cells provided by acCELLerate



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