Collaborative Activities with Anaxon

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2018 - Investigating pain pathways by inhibition of voltage-gated sodium channels

icon sp96   SyncroPatch 384PE and   icon pl   Patchliner poster, FENS Meeting 2018  logo pdf   (2.5 MB)

2017 - Cardiomyocytes in Voltage Clamp and Current Clamp by Automated Patch Clamp

icon sp96   SyncroPatch 384PE and   icon pl   Patchliner poster, BPS Meeting 2017  logo pdf   (1.7 MB)

NaV1.7 - Pharmacology of Lidocaine

icon sp96   PE NaV1.7 LidocaineSyncroPatch 384PE data and applications:
Cells were kindly provided by Anaxon.

The effect of different concentrations of Lidocaine on NaV1.7 currents were investigated. Measured on the SyncroPatch 384PE the whole cell patch methodology and single-hole chips were used. The success rate of > 90% for cells which had a seal resistance > 500 MΩ was determined. A value of 0.79 was calculated for the z-factor (characterization of HTS screening assay quality).

NaV1.7 - Frequency Dependent Inhibition

icon sp96   PE NaV1.7 TetracaineSyncroPatch 384PE data and applications:
Cells were kindly provided by Anaxon.

The frequency dependent inhibition of Tetracaine (5 µM, 15 µM and 50 µM) on NaV1.7 currents were investigated. Measured on the SyncroPatch 384PE the whole cell patch methodology and single-hole chips were used. The IC50 value determined from the first pulse of the pulse train was calculated as 41.8 µM, from the second pulse as 9.9 µM and from the 10th pulse as 3.0 µM. 

 

2018 - Biophysical and Pharmacological Characterization of Voltage-Gated Sodium Channels Involved in Pain Pathways

icon sp96   SyncroPatch 384PE Oral Presentation Video

Presenter: 
Dr. Markus Rapedius, Senior Scientist, Nanion Technologies

2017 - HTS in Cardiac Safety

Icon CE   CardioExcyte 96 presentation (slide deck)   logo pdf   (4.0 MB)

NaV1.7 - Accurate Voltage Clamp

SyncroPatch 384PE Nav17 CHO Anaxon 384 raw IV 2

 icon sp96   SyncroPatch 384PE data and applications:
Cells were kindly provided by Anaxon AG.

CHO cells expressing NaV1.7 were used on the SyncroPatch 384PE with a success rate of > 90% for cells which have a seal resistance > 500 MΩ (see inset). A screenshot of the PatchControl 384 software showing current traces in response to a voltage step protocol and the corresponding current-voltage plot.

2016 - Automated Patch Clamp Meets High-Throughput Screening: 384 Cells Recorded in Parallel on a Planar Patch Clamp Module

icon sp96  SyncroPatch 384PE publication in Journal of Lab Automation (2016)

Authors: 
Obergrussberger A., Brüggemann A., Goetze T.A., Rapedius M., Haarmann C., Rinke I., Becker N., Oka T., Ohtsuki A., Stengel T., Vogel M., Steindl J., Mueller M., Stiehler J., George M., Fertig N.

2014 - Automated Patch Clamp Analysis of nAChα7 and NaV1.7 Channels

icon pap  Port-a-Patch and   icon pl   Patchliner publication in Current Protocols in Pharmacology (2014)

Authors: 
Obergrussberger A., Haarmann C., Rinke I., Becker N., Guinot D., Brueggemann A., Stoelzle-Feix S., George M., Fertig N.

NaV1.7 - "Characterization of hNaV1.7 on Nanion's SyncroPatch 384PE"

icon sp96   SyncroPatch 384PE application note   logo pdf   (0.7 MB)
Cells were kindly provided by Anaxon.

NaV1.7 - "Pharmacology on hNaV1.7 performed on Nanion’s Patchliner at Vhalf "

icon pl   Patchliner application note:   logo pdf   (0.4 MB)
Cells were kindly provided by Anaxon.

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