Port-a-Patch application note: (0.7 MB)
Cells were kindly provided by Millipore.
The gene CACNA1H encodes the α1H subunit of the voltage-gated calcium channel CaV3.2. It belongs to the low voltage-activated T-type calcium channels. CaV3.2 displays the typical characteristics of the T-type channels: activation at low depolarization of the membrane and transient kinetics. T-type Ca2+ channels are involved in diverse, mainly rhythmic processes like e.g. pacemaking and generation of thalamocortical rhythms in sleep or epilepsy. CaV3.2 is expressed in a wide variety of cells. Amongst others it has been found in kidney, smooth muscle, brain, adrenal and cardiac cells. It seems to be involved in contraction of smooth muscle and the secretion of the adrenal hormones aldosterone and cortisol. Pharmacological block of T-type channels may lead to new drugs for the treatment of hypertension and epilepsy. The biophysical and pharmacological properties of the cells are presented in this Application Note.