Patchliner application note: (0.6 MB)
Cells were kindly provided by Millipore.
Acid-sensing ion channels (ASICs) are ligand-gated ion channels activated by protons and are members of the sodium-selective cation channels belonging to the epithelial sodium channel/degenerin (ENaC/DEG) family. They are highly sensitive to extracellular acidosis. In rodents, where they are mainly expressed in neurons of the peripheral nervous system, ASIC3 plays an important role as sensor of non-adaptive pain which is correlated to tissue acidosis. However, the role of the human ASIC3 channel has not yet been elucidated. In contrast to other ASIC ion channels, ASIC3 shows a sustained window current upon external acidification. Here we present data recorded on an 8-channel Patchliner. Current responses upon external acidification as well as amiloride block of acidosis-induced currents of hASIC3-expressing HEK293 cells are shown.